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Method for simultaneously detecting a plurality of mycotoxins in sesame paste

A technology for mycotoxins and sesame paste, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of not being able to detect all mycotoxins, the complex matrix of sesame paste, and the simultaneous detection of sesame paste, etc., to save detection costs and achieve high sensitivity , good repeatability

Active Publication Date: 2015-04-22
PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Claims
  • Application Information

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Problems solved by technology

The matrix of tahini is complex, and it is impossible to detect all kinds of mycotoxins with existing methods, and there is no method for simultaneous detection of multiple mycotoxins in tahini

Method used

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  • Method for simultaneously detecting a plurality of mycotoxins in sesame paste
  • Method for simultaneously detecting a plurality of mycotoxins in sesame paste
  • Method for simultaneously detecting a plurality of mycotoxins in sesame paste

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Embodiment 1

[0034] The detection of the standard addition recovery rate of 26 kinds of standard mycotoxins in embodiment 1 sesame paste

[0035] Including: (1) Sample preparation: add the following 26 mycotoxin standard substances to sesame paste: aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1, AFM2), fumonisins (FB1, FB2, FB3), new Solanum solani fusarium enol (NEO), diacetyl canegrass fusarenol (DAS), fumagillin (FUM), gliotoxin (GLT), Aspergillus versicolor (ST), T2 toxin (T2), HT2 toxin (HT2), verrucous verrucosin (VER), ochratoxin A (OTA), zearalenone (ZEN), deoxynivalenol (DON), fusarenone ( FUS-X), de-epoxy-deoxynivalenol (DOM-1), mycophenolic acid (MPA), microcystin (PAX), 3-acetyl deoxynivalenol (3 -ADON) and 15-acetyldeoxynivalenol (15-ADON) to prepare a single spiked mycotoxin sample with 2.5 g of tahini, the amount of each of the 26 mycotoxins added , that is, the spiked level (Spiked level) is shown in Table 3 below; in addition, 26 kinds of mycotoxin standard substances were add...

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Abstract

The invention relates to a method for detecting a plurality of mycotoxins in a sesame paste, and particularly relates to a method which carries out pre-treatment by an optimized dispersive solid-phase extraction method (QuEChERS), and simultaneously detects 26 mycotoxins in sesame paste by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). The method particularly comprises: extracting a sample twice with acid-containing acetonitrile-aqueous solutions with different concentrations, adding magnesium sulfate and sodium chloride into the sample extract for salting out, performing centrifugation, adding n-hexane into the obtained supernatant, performing vortex oscillation and degreasing, purifying the product with C18 and magnesium sulfate, performing vacuum concentration, dissolving the residues again with methanol and water in order to obtain a sample analytic solution, and detecting the solution by a multi-reaction monitoring mode of ultra-high performance liquid chromatography-tandem mass spectrometry. The method improves the detection efficiency, saves detection cost, is high in sensitivity, high in repeatability, and high in adding standard recovery rate, and can be extended to detection of other samples with a high grease content.

Description

technical field [0001] The invention relates to a method for detecting various mycotoxins in sesame paste, in particular to a method for pretreatment using dispersive solid-phase extraction (QuEChERS) and simultaneous detection using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS) The method of 26 kinds of mycotoxins in sesame paste belongs to the field of food safety testing. Background technique [0002] Mycotoxins are toxic metabolites produced by toxin-producing molds under suitable environmental conditions, and have been a huge potential threat to humans, animals and plants since ancient times. In 1960, more than 100,000 turkeys were poisoned and died in the United Kingdom. Studies have found that moldy peanut cake powder imported from Brazil was included in the feed. Feeding rats with this peanut cake powder can induce liver cancer and substances that induce liver cancer It was confirmed to be a metabolite of Aspergillus flavus, nam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘萤韩深张锡全王珮玥王金花程甲
Owner PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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