30 results about "Fumagillin" patented technology

The invention discloses a seed medium for producing fumagillin by fermentation of Aspergillus fumigatus and its fermentation medium, and also discloses a method for producing fumagillin by fermentation of Aspergillus fumigatus. The Aspergillus fumigatus is inoculated in the seed medium to activate the strain, and the pH value of the medium is natural; step 2, the activated strain is prepared to prepare a monosporous bacteria suspension and inoculate the fermentation medium, and the pH of the fermentation medium is When the value is natural, the fermentation culture is carried out, and the fumagillin in the fermentation broth is collected. The present invention screened the carbon sources affecting fumagillin, and unexpectedly found that the use of glycerol as the carbon source could avoid the production of by-products such as gliotoxin, without the need for subsequent separation and purification, reducing production costs and facilitating large-scale production in factories .

The invention discloses a production method of a traditional Chinese medicine preparation toxin remover for preventing and treating mycotoxicosis. The traditional Chinese medicine preparation toxin remover is characterized by comprising the following raw materials by weight percent: 7-9% of folium artemisiae argyi, 6-8% of mother chrysanthemum, 7-9% of dandelion, 7-9% of fordia cauliflora hemsl, 6-8% of oriental wormwood, 7-8% of sweet wormwood, 7-9% of derris eriocarpa how, 7-9% of sticktight, 5-7% of semen plantaginis, 6-8% of black nightshade, 6-7% of azadirachta indica and 8-9% of dried radix rehmanniae. The production method comprises the following steps: A, respectively cleaning, airing and crushing the above traditional Chinese medicine raw materials, sieving by a 300-mesh sieve, so as to obtain traditional Chinese medicine fine powders; B, respectively weighing traditional Chinese medicine fine powders according to the ratio, and mixing and stirring well; C, adding a proper amount of auxiliary material to dilute, and mixing well, so as to obtain a brown solid powdery product. The production method has the beneficial effects that the traditional Chinese medicine preparation toxin remover has specific adsorption capacity, can efficiently remove various mycotoxins in a target manner, and has a good effect on zearalenone, vomitoxin, aflatoxin, ochratoxin, fumagillin, T-2 toxin and the like in an oriented mode; the detoxification ability of the liver on the toxins can be improved; and the damage to an animal body caused by the toxins can be removed.

The invention discloses a new Penicillium sp. HS‑NF‑684Z, the preservation number is: CGMCC No.14144; meanwhile, it discloses the preparation of fumagillin or a drug combination containing fumagillin by culturing it way of things.

The invention relates to a method for producing fumagillin by aspergillus fumigatus. The method comprises the following steps: inoculating aspergillus fumigatus into a GMM solid medium to activate strains, wherein the pH value of the GMM medium is 5 to 7; preparing activated strain spores into spore suspension, and then inoculating the spore suspension into a CYA solid medium, a CXMA solid medium or an LMM liquid medium, wherein the pH values of the CYA solid medium and the CXMA solid medium are natural, and the pH value of the LMM liquid medium is adjusted to be 5 to 7, and carrying out culturing at the temperature of 25 to 37 DEG C; collecting cultured aspergillus fumigatus to extract fumagillin and fumagillin derivatives. According to the invention, carbon and nitrogen sources and trace elements of fumagillin which affect the growth of aspergillus fumigatus are aimed to be screened, and the concentration of corresponding trace elements is optimized to obtain higher yield of fumagillin.

The present disclosure provides a fumagillol-type compound and its use in the treatment of medical conditions such as obesity. Pharmaceutical compositions and methods, eg, for treating obesity, are provided.

Methionine aminopeptidases catalyse the co-translational removal of amino terminal methionine residues from nascent polypeptide chains. A newly-discovered enzyme, designated methionine aminopeptidase type-3 (MetAP-3), has a substrate specificity which is similar to MetAP-1 and MetAP-2, although it is not inhibited by fumagillin, an irreversible inhibitor of MetAP-2. MetAP-3 also preferentially localizes to mitochondria, unlike MetAP-1 and MetAP-2, which accumulate in the cytoplasm. One embodiment of the present invention relates to human cDNAs encoding polypeptides comprising MetAP-3. Other embodiments of the invention relate to nucleic acid molecules derived from these cDNAs, including complements, homologues, and fragments thereof, and methods of using these nucleic acid molecules, to generate polypeptides and fragments thereof. Other embodiments of the invention relate to antibodies directed against polypeptides comprising MetAP-3, and methods to screen for compounds or compositions that preferentially or specifically effect the activity of polypeptides comprising MetAP-3.

The invention relates to an irradiation degradation processing method of fumitremorgin and T-2 toxin. The irradiation degradation processing method comprises the steps of (1) preparing standard solutions for standard substances of fumitremorgin FB1 and the T-2 toxin, carrying out irradiation processing with the irradiation range of 0-200kGy on the standard solutions, and measuring the contents of the FB1 and T-2 through a liquid chromatogram-tandem mass spectrometry; (2) carrying out preprocessing on samples containing poison after irradiation with the irradiation range of 0-9kGy, and measuring the FB1 and T-2 degrading effects through a liquid chromatogram-tandem mass spectrometry; (3) selecting the FB1 and T-2 standard solutions with highest concentrations as the samples to carry out irradiation processing with the irradiation range of 0-200kGy, and then detecting and analyzing degradation products through the liquid chromatogram-tandem mass spectrometry. The irradiation degradation processing method provided by the invention has the advantages of realizing research and environment-friendly application of the FB1 and T-2 degradation product which lacks of irradiation degradation, the degradation effect is good, and the environment-friendly detoxification processing of mildew agricultural products waste is realized.

The invention provides an extraction method of fumagillin.The method comprises the steps that 1, the pH value of fumagillin fermentation liquor is adjusted to be 1-6.0 and adsorbed with macroporous resin; 2, the resin adsorbing the fermentation liquor is put into a chromatographic column and cleaned with ethanol water with the mass concentration of 15%-30%; 3, the resin is eluted with absolute ethyl alcohol or ethanol water with the mass concentration of 50%-98%, eluent is concentrated and dried, and a coarse fumagillin extraction product is obtained.According to the extraction method, the processes such as various solvent extraction and back extraction in a traditional technology are omitted, the operational reliability is high, simpleness and convenience are achieved, the production process is safe and free of pollution, the resource utilization rate is high, the production cost is low, environmental friendliness is facilitated, and the extraction method is a green and environment-friendly method for extracting the fumagillin.

The invention relates to the field of compounds and in particular to amino-polyols substituted fumagillin as well as a synthetic method and application thereof. The amino-polyols substituted fumagillin has a structure as shown in a formula I. A novel derivative of the fumagillin provided by the invention (namely amino-polyols substituted fumagillin or fumagillin amino-polyols) has excellent water solubility and stability. When the preparation disclosed by the invention and common fumagillin bicyclohexane salts are used for treating ill swarm which is nearly consistent with Nosema apis under the equal concentration, the treatment effect of the amino-polyols substituted fumagillin disclosed by the invention is basically consistent with the effect of common fumagillin bicyclohexane salts. Because any bicyclohexane residue does not exist in the amino-polyols substituted fumagillin, the risk of bees and human health is reduced. Moreover, the product is high in solubility and stability. The structural formula is as shown in the specification.