Method for screening sweetening agent in milk and dairy products by applying ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry

Abstract

The invention discloses a method for screening a sweetening agent in milk and dairy products by applying ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry, and belongs to the technical field of food safety inspection. An optimized QuEChERS method is used for preprocessing samples; the ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry is applied for performing screening, a scanning mode of full-scanning / data-dependency collection and full-scanning / variable data-independent collection and full-scanning / full-ion fragmentation collection is adopted, a positive ion or negative ion mode is selected for scanning, and complete first-order and second-order spectra are obtained; Extract Finder 2.5 software is applied for processing the obtained first-order and second-order spectra, and information of a compound is extracted. Information in a built spectrum library and a tested object screening result are compared, and the composition of the sweetening agent in the milk and dairy products is ensured. An adopted mass spectrometer has the main advantages that the resolution is high, qualitative and quantitative results are accurate, sensitivity is high, and quality precision is high and can be applied to complex matrix analysis. The established screening method has the good application prospect in the field of food.

Description

[0001] 【Technical Field】 [0002] The invention belongs to the technical field of food safety detection, and particularly relates to an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for sweeteners in milk and dairy products. [0003] 【Background technique】 [0004] Sweeteners refer to food additives that can impart sweetness to foods. It is the largest and most commonly used type of additives in foods. There are 20 kinds of sweeteners listed in Appendix A of GB2760. Sweeteners can be divided into nutritive sweeteners and non-nutritive sweeteners according to their nutritional value; according to their sweetness, they can be divided into low power sweeteners. And high-strength sweeteners; according to its source, it can be divided into natural sweeteners and synthetic sweeteners. Add appropriate amount of sweeteners such as aspartame, acesulfame potassium, sodium saccharin to milk and dairy products to ...

AI Technical Summary

Problems solved by technology

[0005] However, at present, there are few high-throughput and rapid detection methods for exogenous risk substances in milk and dairy products. The pre-treatment operations are complicated, the cost of materials and equipment is high, and they are limited to the rapid detection of several or one type of compounds. During rapid analysis, compounds are often grouped according to their structural characteristics and then detected

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