Method for detecting tobacco-specific nitrosamines

A detection method and technology for nitrosamines, which are used in measuring devices, instruments, scientific instruments, etc., can solve the problems of few extraction times, high sample processing throughput, short analysis time, etc., and achieve low reagent consumption and simple pretreatment. , Pre-processing simple effects

Active Publication Date: 2011-04-13
HONGTA TOBACCO GRP
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned existing tobacco-specific nitrosamine determination method complex, time-consuming and other problems, the purpose of the present invention is to provide a highly efficient and sensitive tobacco-specific nitrosamine detection method, each sample of the method only needs to capture relatively Less total particulate matter in cigarettes, fewer extraction times, shorter analysis time for each sample, high isotope internal standard quantitative sensitivity, and high sample processing throughput, suitable for accurate testing of large batches of samples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: After the flue-cured sample cigarettes were equilibrated for 48 hours at a temperature of 22°C±1°C and a relative humidity of 60±2%, a Cambridge filter was used to collect the total particulate matter in the mainstream smoke of 4 cigarettes. According to the method described in the above-mentioned technical scheme of the present invention, repeat determination three times. The measured contents of TSNAs in the total particulate matter of mainstream smoke of flue-cured samples were: NNN2.24ng / cig, NAT15.72ng / cig, NAB1.35ng / cig, NNK3.82ng / cig.

Embodiment 2

[0026] Example 2: The method for measuring TSNAs in the mainstream smoke of mixed sample cigarettes is the same as that in Example 1. The measured content of TSNAs in the total particulate matter of mainstream smoke of mixed sample cigarettes were: NNN32.12ng / cig, NAT53.63ng / cig, NAB6.14ng / cig, NNK22.91ng / cig.

Embodiment 3

[0027] Example 3: After the flue-cured sample cigarettes were equilibrated for 48 hours at a temperature of 22°C±1°C and a relative humidity of 60±2%, the total particles in the sidestream smoke of 2 cigarettes were collected with a Cambridge filter and a fishtail hood Xiangwu. After the fishtail mask was eluted with 10 mL of ammonium acetate aqueous solution containing the internal standard, the eluate was added to the Erlenmeyer flask containing the Cambridge filter. According to the method described in the above-mentioned technical scheme of the present invention, repeat determination three times. The measured contents of TSNAs in total particulate matter in sidestream smoke of flue-cured samples were: NNN18.61ng / cig, NAT23.84ng / cig, NAB5.92ng / cig, NNK127.03ng / cig.

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Abstract

The invention relates to a method for measuring tobacco-specific nitrosamines (TSNAs for short) by using a super efficient liquid chromatogram-tandem mass spectrum combined method, and belongs to a method for detecting TSNAs. The method comprises the following steps of: adding internal standard-containing aqueous solution of ammonium acetate into a filter sheet after trapping smoke and cigarette filters smoked completely under standard conditions or tobacco shreds, performing ultrasonic extraction, transferring 1 to 2mL of extract into a small solid-phase extraction column taking N-vinyl pyrrolidone-benzene sulfonic acid copolymer as filler, flushing the small column by using aqueous solution of methanoic acid and methanol, finally eluting the small column by using solution of aqueous ammonia and methanol solution, analyzing the eluant by using an ultra performance liquid chromatography-mass spectrometer/mass spectrometer (UPLC-MS/MS), and quantifying according to the area ratio of a component peak to an isotope internal standard peak. The detecting method can be used for detecting the TSNAs in cured and hybrid cigarette mainstream smoke, cigarette side-stream smoke, filter tip intercepting smoke and tobacco shreds, and has the advantages of simple pretreatment, accurate quantification, high instrument analysis speed, low detection limit, high sample treatment flux and the like.

Description

technical field [0001] The invention relates to an efficient and sensitive detection method for tobacco-specific nitrosamines. technical background [0002] Tobacco-specific nitrosamines (TSNAs) are a class of nitrosamines with the most abundant N-nitrosamine content in tobacco, which only exist in tobacco, tobacco products and cigarette smoke. hazards are important. The results of the toxicity evaluation of the harmful ingredients in the Canadian government list by the International Agency for Research on Cancer (IARC) show that the pure chemical ingredients of NNK and NNN are suspected human carcinogens. In order to reduce the possible harm of tobacco-specific nitrosamines to the health of smokers and passive smokers, controlling their content in tobacco and tobacco products has become an important issue. Therefore, accurate determination of TSNAs in tobacco and tobacco products will help to provide a reliable basis for correctly evaluating the health hazards of smoking ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张凤梅杨柳汤建国吴亿勤孟昭宇
Owner HONGTA TOBACCO GRP
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