34 results about "Chicken sample" patented technology

The invention discloses a method for identifying the variety of large bone chicken by using an enzyme digestion technique and a special primer pair for PCR amplification. Special primers are shown in the sequence list SEQ ID NO 1-2. The method for identifying the variety of the large bone chicken comprises the following steps that PCR amplification and enzyme digestion are simultaneously conducted on large bone chicken sample DNA and a to-be-tested DNA mixed sample by using the special primer pair for PCR amplification; then enzyme-digested products are tested by adopting 2.0% agarose gel electrophoresis, and two electrophoresis strips (294bp, 460bp) in the agarose gel electrophoretogram of the large bone chicken sample DNA serve as a positive result; an enzyme-digested product agarose gel electrophoretogram of the to-be-tested DNA mixed sample is compared with the positive result, and if two electrophoresis strips (294bp, 460bp) appear in the enzyme-digested product agarose gel electrophoretogram of the to-be-tested DNA mixed sample, the to-be-tested sample is identified as a pure variety of the large bone chicken. According to the method for identifying the variety of the large bone chicken by using the enzyme digestion technique and the special primer pair for PCR amplification, PCR amplification and enzyme digestion test is conducted on the to-be-tested sample by using an SNP variable site, detected in a laboratory, in a d-loop region of the large bone chicken, and the designed primers and enzyme-digestion enzyme have good specificity.

The invention discloses an identification method of the meat quality of a yellow feather broiler and a white feather broiler. The identification method is respectively used for measuring the protein content, the fat content, the brightness value, the hardness value and the cooking loss ratio of a to-be-measured chicken sample. Measured numerical values are input into an identification equation to be calculated to obtain a Y value, if the Y value is less than or equal to 26.65, the to-be-measured chicken sample is determined to be the yellow feather broiler, and if the Y value is greater than or equal to 26.89, the to-be-measured chicken sample is determined to be the white feather broiler, wherein the identification equation is as follows: Y=(0.2127*the protein content)+(0.1196*the fat content)+(0.0522*the brightness value)+(0.3534*the hardness value)+(0.2620*the cooking loss ratio). The identification method has the advantages of simplicity, easiness in operation, high accuracy and practicability, and the like.

The invention discloses a rapid detection method based on Raman spectrum inosinic acid, an umami substance in fresh chicken, and relates to the technical field of poultry meat quality and safety detection. The method is as follows: ① Determining the Raman spectrum of a standard inosinic acid; ② Obtaining the Raman spectrum of a fresh chicken sample; ③ Determining the content of inosinic acid by a chemical method; ④ Correcting the Raman spectrum; ⑤ Optimizing the characteristic peak of inosinic acid; ⑥Calculate the characteristic Raman peak area; ⑦Establish the detection model of inosinic acid content. The present invention quickly detects the umami substance inosinic acid in fresh chicken through the microscopic confocal Raman spectroscopy technology, and finds an effective, simple operation, less cost and time-consuming chicken umami substance inosinic acid rapid detection method. Detection method; the detection method is simple, safe and efficient, can meet the needs of industrial production and consumers' requirements for product quality, and meet the needs of evaluating the flavor quality of chicken products in the actual production and processing process.