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Method for quickly detecting chicken enterobacteriaceae content in online mode

A chicken enterobacteriaceae content technology, applied in the direction of measuring devices, scientific instruments, material analysis through optical means, etc., can solve the difficult fast and non-destructive detection requirements, hidden dangers of consumer health problems, long cultivation cycle and other problems, to achieve Achieve large-scale online detection, realize large-scale online detection, and improve the effect of accuracy

Inactive Publication Date: 2018-11-23
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the content of Enterobacteriaceae in chicken reaches a certain value, once consumers eat this kind of chicken by mistake, it will bring hidden dangers to consumers' health problems
The current national standards for the detection of Enterobacteriaceae in food are based on GB4789.3-2016 "Food Microbiological Examination of Coliform Bacteria Counting", but this method has the disadvantages of cumbersome steps, long cultivation period, high cost and damage to samples, which is difficult Realize the modern meat industry's requirements for fast and non-destructive testing
[0003] The hyperspectral imaging technology, which combines traditional spectral technology and image technology, can simultaneously capture the spectral and image information of the measured sample, and has the characteristics of no preprocessing, non-destructive, fast, easy to operate and low cost, etc. A lot of research has been done on the non-destructive testing of meat products, but less research has been reported on Enterobacter chicken

Method used

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  • Method for quickly detecting chicken enterobacteriaceae content in online mode
  • Method for quickly detecting chicken enterobacteriaceae content in online mode
  • Method for quickly detecting chicken enterobacteriaceae content in online mode

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Embodiment

[0018] A kind of fast nondestructive on-line detection method step of chicken Enterobacteriaceae content of the present embodiment is as follows:

[0019] (1) Divide the purchased whole fresh chicken breast into small samples of 3cm*3cm*1cm in the laboratory. A total of 102 small samples are obtained, which are called calibration sets, and then divide them into 7 parts on average, and put them into the Put it in a disposable plastic box, put it in a refrigerator at 4°C for refrigeration, and take out a copy at 0, 1, 2, 3, 4, 5, and 6 days for testing;

[0020] (2) Before the test, the hyperspectral imaging system was turned on to preheat 30 minutes in advance, and the chicken sample was also taken out of the refrigerator 30 minutes in advance. When the optimal spectral image acquisition speed is 6.54mm / s and the exposure time is 4.65ms, then save the blackboard and whiteboard images, and finally collect the sample images;

[0021] (3) Immediately detect the number of enteroba...

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Abstract

The invention discloses a method for quickly detecting a chicken enterobacteriaceae content in an online mode. The method comprises the steps of acquiring a hyperspectral image of a calibration set chicken sample, pretreating the acquired spectral image, then recognizing a target area and extracting average spectral data of the spectral image; substituting the extracted spectral data into a formula. According to the method disclosed by the invention, 17 optimal wavelengths are extracted from 484 full wavebands, so that a lot of redundant information is removed, useful information is extracted,and the calculated amount of data analysis is reduced; thus, accuracy of a partial least squares model is improved, and a large-scale online production requirement of a meat enterprise is achieved. Compared with the prior art, the method disclosed by the invention has the following beneficial effects that a sample to be detected is prevented from being pretreated, only non-contact spectrum scanning is performed on the sample, and no destructiveness is generated to the sample; no chemical reagent is used by the method disclosed by the invention, so that greenness is achieved, and cost is reduced; the method disclosed by the invention is easy to operate, can save time and can achieve large-scale online detection on the chicken enterobacteriaceae content.

Description

technical field [0001] The invention relates to the field of food quality and safety detection, in particular to a method for quickly detecting the content of Enterobacteriaceae in chicken on-line. Background technique [0002] Among the many meat products today, chicken is suitable for a variety of cooking methods. Its meat is tender, delicious, and rich in various nutrients, so it has the effect of nourishing and nourishing the body. Chicken, cures nausea, abdominal pain, bone pain, mastitis, anti-abortion" and other effects, and is very popular among consumers. Chicken is a food that is highly susceptible to contamination by endogenous and exogenous microorganisms, and as the microbial content increases sharply, the freshness of the chicken will also decrease. At present, Enterobacteriaceae is used as one of the indicators of food hygiene in my country, and Enterobacteriaceae is also one of the must-test items for quality control in international food microbiology labora...

Claims

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 何鸿举马汉军王慧朱明明王正荣
Owner HENAN INST OF SCI & TECH
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