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Method for separating and purifying lectin from pleuratus ferulae

A technology for separation and purification of lectins, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as complex process, low efficiency of lectin separation and purification, high production and use costs, and achieve technological progress Simple, strong promotion and application value, low production and use costs

Inactive Publication Date: 2013-09-04
SHIHEZI UNIVERSITY
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Problems solved by technology

[0004] The present invention provides a method for separating and purifying lectin from ferulae ferulae, aiming to solve the complicated technological process of separating and purifying lectin from ferulae ferulae provided by the prior art, the separation and purification efficiency of lectin is low, and the production and use high cost problem

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  • Method for separating and purifying lectin from pleuratus ferulae

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Embodiment Construction

[0016] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the invention.

[0017] figure 1 It shows the implementation process of the method for the separation and purification of Ferula lectin provided by the embodiment of the present invention.

[0018] The method includes the following steps:

[0019] In step S101, the phosphate buffer solution was leached overnight, and the supernatant was collected by centrifugation;

[0020] In step S102, use 40-70% ammonium sulfate for precipitation, centrifuge to remove the precipitate, dialyze to remove ammonium sulfate, and freeze-dry;

[0021] In step S103, pass the dissolved supernatant through a DEAE-52 ion-exchange chromatogr...

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Abstract

The invention belongs to the technical field of separation and purification of lectin, and provides a method for separating and purifying lectin from pleuratus ferulae. Immersion is performed with normal saline overnight, a supernatant is collected centrifugally, 40%-70% of ammonium sulfate precipitation is adopted, the precipitation is removed centrifugally, ammonium sulfate is removed by dialysis, freeze drying is performed, the dissolved upper-layer clear liquid passes through a DEAE-52 ion-exchange chromatographic column, an elution peak is collected, freeze drying is performed, the dissolved upper-layer clear liquid is loaded through SaphedexG-100, and the elution peak is collected, and the procoagulant activity is detected. A technological process for separating and purifying lectin from pleuratus ferulae is simpler and more convenient, the separation and purification efficiency of the lectin is higher, the production and usage costs are lower, the practicability is high, and higher popularization and application values are provided.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of lectin from Ferulicum ferulae, and in particular relates to a method for separation and purification of lectin from Ferulae ferulae. Background technique [0002] Lectins have a variety of biological activities and are widely used in medicine, food, fresh-keeping and other industries. Lectins have dual regulatory effects on the body’s immunity and can promote the body’s recognition and elimination of tumor cells. Lectins acting alone on tumor cells can cause Tumor cell apoptosis also has a good inhibitory effect on virus replication in vivo, and can also promote the recovery process of the body after inflammation. [0003] The process for separating and purifying lectins from Ferula aeruginosa provided by the prior art is relatively complicated, the separation and purification efficiency of lectins is low, and the production and use costs are relatively high. Contents of t...

Claims

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Application Information

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IPC IPC(8): C07K14/37C07K1/36C07K1/30C07K1/18
Inventor 许程剑王月香闫妮娜李应彪卢士玲孙然然牛博楠
Owner SHIHEZI UNIVERSITY
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