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Application of wheat germ agglutinin in the preparation of products for inhibiting coronavirus

A technology of wheat germ agglutinin and coronavirus, applied in antiviral agents, medical preparations containing active ingredients, and resistance to vector-borne diseases, etc., can solve the problems that have not yet been applied to wheat germ agglutinin, and achieve good application value Effect

Active Publication Date: 2022-03-08
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no application of wheat germ agglutinin in the treatment of infections caused by coronaviruses SARS-CoV, MERS-CoV, SARS-CoV-2 and SARS-CoV-2 mutants

Method used

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  • Application of wheat germ agglutinin in the preparation of products for inhibiting coronavirus
  • Application of wheat germ agglutinin in the preparation of products for inhibiting coronavirus
  • Application of wheat germ agglutinin in the preparation of products for inhibiting coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Phytohemagglutinin detection of SARS-CoV-2 S protein binding activity and antiviral activity

[0083] 1. Experimental materials

[0084] Lentil lectin, wheat germ agglutinin (WGA), sessile elderberry agglutinin (SSL), peanut lectin (Peanut lectin), Huaihuai agglutinin (MAL) were purchased from Wako (Japan) Datura agglutinin (DSL), succinyl-concanavalin A (succ-Con A), galactus agglutinin (GNL), phytohemagglutinin (PHA-E), leukolectin (PHA-L ), phytohemagglutinin-M (PHA-M) and phytohemagglutinin-P (PHA-P) from red kidney bean were purchased from Sigma Aldrich; SARS-COV-2 S trimer expressed in HEK293 cells was purchased from at ACRO Biosystems (China, Beijing); 293T cells were provided by the American Type Culture Collection (ATCC); Huh7 cells were provided by the Japanese Research Biological Resource Collection (JCRB); the SARS-CoV-2spike (Wuhan-Hu- 1, MN908947; hCoV-19 / South Africa / KRISP-K007869 / 2020, B.1.351, EPI_ISL_860630; hCoV-19 / England / QEUH-F56F0F / 2021...

Embodiment 2

[0097] Example 2 Detection of the Inhibitory Effect of Plant Hemagglutinin on the Infection of SARS-CoV-2 S Protein Mutants and SARS-CoV-2 Epidemic Mutants

[0098] In this example, the inhibitory effects of various plant lectins on the infection of SARS-CoV-2 S protein mutant strains and SARS-CoV-2 epidemic mutant strains, including the British epidemic mutant strain B.1.1.7, were detected. , South African popular mutant strain B.1.351, Brazilian popular mutant strain P.1, combined mutant strain D614G+I472V, combined mutant strain D614G+A435S, A222V, K417N, S477N, E484K, N501Y, combined mutant strain A222V+D614G, combined mutant strain Mutant strains such as strain K417N+D614G, combined mutant strain S477N+D614G, combined mutant strain E484K+D614G, combined mutant strain N501Y+D614G;

[0099] Lectins are mainly aimed at the glycosyl part of glycoproteins. In this example, the inventors studied whether the change of the glycation site of SARS-CoV-2 S protein would affect its n...

Embodiment 3

[0109] Example 3 Detection of Hemagglutination Activity and Cytotoxicity of Plant Hemagglutinin

[0110] Since many natural lectins can cause hemagglutination, in this example, the inventors studied the hemagglutination activity of different lectins, and studied the cytotoxicity of Huh7 and 293T cells.

[0111] 1. Detection of hemagglutination activity of phytohemagglutinin

[0112] Chicken blood samples were used to detect the hemagglutination activity of phytohemagglutinin, the rooster red blood cells were washed with PBS, and resuspended at a final concentration of 1% (v / v), and 50 μL of 2-fold serially diluted phytohemagglutinin solution was mixed with an equal volume of red blood cells The solution was mixed in a 96-well round-bottom plate, and a 2-fold serial dilution of influenza antigen (B / Maryland / 15 / 2016, NIBSC-UK-EN63QG, NIBSC code: 18 / 104, HA: 69 μg / mL) was performed and served as a positive control , PBS solution was used as a negative control, the plate was incu...

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Abstract

The invention discloses the application of wheat germ agglutinin in the preparation of products for inhibiting coronaviruses. The invention finds for the first time that the wheat germ agglutinin is effective against SARS‑CoV, MERS‑CoV, SARS‑CoV‑2 and SARS‑CoV‑2 mutant strains. It has good antiviral activity, and also shows certain antiviral activity to vesicular stomatitis virus (VSV), which can be used to treat and prevent infection caused by coronavirus, and can be used as a candidate drug for the prevention and treatment of coronavirus infection. Good application value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of wheat germ agglutinin in the preparation of products for inhibiting coronavirus. Background technique [0002] Coronaviruses (Coronaviruses, CoVs) are one of the main viral pathogens that cause respiratory tract infections (Fehr A R, Perlman S.Coronaviruses: an overview of their replication and pathogenesis [J]. Coronaviruses, 2015: 1-23.), coronaviruses belong to Nested virus order, Coronaviridae, coronavirus genome and subgenome contain at least 6 open reading frames (Open reading frame, ORF), generally have 5' cap (Leader) and 3' end (Ends) sequence, 5' The cap open reading frame codes to produce a variety of non-structural proteins, which are involved in the transcription and replication of the virus; coronaviruses have at least 4 main structural proteins, including the spike protein (Spike protein, S protein), the envelope protein (Envelope...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P31/14A61P31/12A61P31/22
CPCA61K38/168A61P31/14A61P31/12A61P31/22Y02A50/30
Inventor 王佑春王兰黄维金王文波李倩倩吴佳静武刚于传飞郭璐韵郭莎
Owner NAT INST FOR FOOD & DRUG CONTROL
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