The invention discloses a reagent kit for detecting novel coronaviruses and influenza viruses at the same time. The reagent kit disclosed by the invention specially comprises a primer probe for separately targeting S genes, E genes, N genes and / or ORF1ab genes of novel coronaviruses, and a primer probe for targetting characteristic consensus sequence of the influenza viruses A / B, and novel coronavirusRNA for qualitatively detecting pneumonia suspected cases and suspected clustered cases of patients infected with the novel coronaviruses, or other novel coronavirusRNA for diagnosing infectionof the novel coronaviruses or identifying novel coronavirusRNA in other samples of nasopharyngeal swabs, sputamentum and the like of diagnostician, and besides, the reagent kit is used for detectingRNA of influenza viruses A / B in the samples to assist in elimination of suspected cases, can be used for identifying and diagnosing infection with the novel coronaviruses, and is high in sensitivity and convenient to use.
Provided are a detection reagent and a kit. The reagent detects a 2019-coronavirus SARS-CoV-2 immunoglobulin A (SARS-CoV-2 IgA) antibody in blood and a salivary liquefied sugar chain antigen KL-6 at the same time, and is used for screening, especially for early screening of 2019-coronavirus infected pneumonia, and for pre-judging the detection reagent for severe patients. The kit comprises a testcard, and the test card is composed of an SARS-CoV-2 IgA test strip, a KL-6 test strip and a plastic box. The reagent and the kit disclosed by the invention can be used for diagnosing the new coronavirus pneumonia at an earlier stage and can be used for judging whether a patient suffering from the new coronavirus pneumonia has lung injury or not.
The present invention provides a method for treating coronavirus infections in cats or other animals by administering a nucleoside represented by a formula (I) and a corresponding prodrug, solvate, orpharmaceutically acceptable salt thereof, a pharmaceutical composition comprising the compound, wherein the nucleoside and the prodrug thereof in the formula (I) are in alpha configuration, and R1, R2, R3 and R4 are all defined in the specification. The formula (I) is defined in the specification.
The invention provides a novel coronavirus detection test strip as well as a preparation method and application thereof. The test strip comprises a binding pad and an analysis membrane, and the binding pad is coated with a luminescent substance labeled 2019-nCoV recombinant antigen and a mouse anti-human HCG monoclonalantibody; a T2 detection line, a T1 detection line and a quality control line are sequentially arranged on the analysis membrane along the chromatography direction; the T2 detection line is coated with a mouse anti-human IgG monoclonalantibody, the T1 detection line is coated with a mouse anti-human IgA monoclonalantibody and a mouse anti-human IgM monoclonal antibody, and the quality control line is coated with a goat anti-mouse IgG polyclonal antibody. The test paper card can simultaneously determine the positive conditions of IgA antibody, IgM antibody and IgG antibody in serum of a patient, can more accurately detect the early antibody level condition in the body of the patient, assists in judging different periods of novel coronavirus infection of the patient, and improves the sensitivity and specificity of novel coronavirus detection.
The invention discloses a novel coronavirusT cellepitopepeptide, pMHC and the preparation and application thereof. The amino acid sequence of the novel coronavirusT cellepitopepeptide is as shown in any one of SEQ ID NO: 1-9. According to the invention, nine novel coronavirusT cellepitope peptides are discovered; the pMHC compound monomer is prepared by using the pMHC compound; the pMHC complex polymer can be used for detecting antigen-specific T cells in peripheral blood of a novel coronavirus infected rehabilitative person and used for an in-vitro T cell activation experiment, and the novel coronavirus T cell epitope peptides can be applied to immunodetection related to novel coronaviruses.