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Polypeptide medicine for inhibiting SARS coronavirus, and derivatives and use thereof

A coronavirus and derivative technology, applied in the direction of viral peptides, biochemical equipment and methods, positive-sense single-stranded RNA viruses, etc.

Inactive Publication Date: 2004-04-14
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report so far to design a polypeptide or its analogues as a drug for preventing and treating coronavirus infection based on this characteristic.

Method used

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  • Polypeptide medicine for inhibiting SARS coronavirus, and derivatives and use thereof
  • Polypeptide medicine for inhibiting SARS coronavirus, and derivatives and use thereof

Examples

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Embodiment

[0019] 1. Prediction of the HR1 and HR2 regions of the SARS coronavirus spike (S) protein The SARS coronavirus S protein sequence used comes from a Toronto isolate, and the GenBank accession number is: NC_004718. The S protein was analyzed using a computer program, indicating that the SARS coronavirus S protein has two typical heptapeptide repeat sequences. At the same time, other different programs were used for analysis. The results showed that the positions of HR1 and HR2 were similar, and finally the HR1 sequence was determined as Seq ID: No.1; HR2 sequence is Seq ID: No.2.

[0020] 2. Artificial synthesis of SARS coronavirus S protein HR1 and HR2 polypeptides

[0021] According to the amino acid sequences of HR1 and HR2 provided in Example 1, the HR2 polypeptide was artificially synthesized, and three HR1 polypeptides of different lengths were synthesized at the same time. The purity was over 90% as identified by HPLC, and the synthesized peptide was very soluble.

[002...

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Abstract

The invention provides a method for blocking SARS coronavirus to infect cell with polypeptide or its derivative. The cistine series of polypeptide comes from two heptad repeat districts HR1 and HR2 of SARS coronavirus spike(S) protein. The invention also provides a method for producing the functional similarity with HR1 and HR2. the invention provides a method for producing HR polypeptide and its derivant with coalescence expression method. All the peptides have rejection capability to SARS.

Description

Field of invention: [0001] The invention belongs to the field of molecular biology of viruses. Specifically, it involves the use of the heptad repeat (HR) HR1 and HR2 polypeptides of the SARS coronavirus spike (S) protein and their derivatives to prevent and treat SARS coronavirus infection. Background technique: [0002] Recently, a contagious severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, SARS) or "atypical pneumonia" broke out all over the world, and its pathogen has been preliminarily identified as a novel coronavirus (Coronavirus). Coronavirus is an enveloped RNA virus. Similar to other enveloped viruses, it needs to release genetic material into the cell through the fusion of the virus envelope and the cell membrane to complete the infection of the cell. Studies on the fusion mechanism of most enveloped viruses have found that they use envelope fusion glycoproteins to mediate the fusion of the viral envelope and the host cell membrane through co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K39/00A61K39/215A61P11/00A61P31/14C07K14/165C12N15/50C12N15/63C12P21/00
CPCC07K2319/00A61K39/00C12N2770/20022C07K14/005A61P11/00A61P31/14
Inventor 高福田波朱杰青肖庚富郑从义
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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