The invention relates to an HIV-1
protease inhibitor
drug resistance mutation detection method and a kit. Specifically, the invention also discloses a probe for detection of
human immunodeficiency virus protease gene drug resistance mutation, and the probe includes a probe for detection of
protease gene D30N
mutation, a probe for detection of
protease gene V32I
mutation, a probe for detection of
protease gene M46I and M46L
mutation, a probe for detection of
protease gene I47V and I47A mutation, a probe for detection of protease
gene G48V mutation, a probe for detection of protease gene I50V mutation, a probe for detection of protease gene I54V mutation, a probe for detection of protease gene V82A, V82F, V82T and V82S mutation, a probe for detection of protease gene I84V mutation, a probe for detection of protease gene N88D mutation, and a probe for detection of protease gene L90M mutation.