Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor

A deubiquitinating enzyme and interferon technology, applied in the field of biomedicine, can solve the problems of few research reports on 3C-like proteases and unclear biological functions

Inactive Publication Date: 2019-09-20
ACADEMY OF MILITARY MEDICAL SCI
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Studies in the prior art have found that papain-like proteases participate in a series of important functions of innate immune response, but there are few reports on 3C-like proteases, and their biological functions are still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor
  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor
  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] This example clarifies the mechanism of 3CLpro regulating host antiviral innate immune response from various aspects and perspectives, and clarifies the use of MERS-CoV 3CLpro as a deubiquitinating enzyme and interferon inhibitor.

[0044] 1 method

[0045] 1.1 Construction of MERS-CoV / SARS-CoV / NL63-CoV 3CLpro and its mutants

[0046] In order to study the structure and biological characteristics of MERS-CoV / SARS-CoV / NL63-CoV, the present invention obtains the MERS-CoV / SARS-CoV / NL63-CoV 3CLpro sequence by comparing and analyzing the genome sequence, and at its C-terminal Connect the V5 sequence, artificially synthesized by GenScript. Using MERS-CoV / SARS-CoV / NL63-CoV 3CLpro as a template, mutants were obtained by site-directed mutagenesis.

[0047] 1.1.1 Amino acid sequence of MERS-CoV / SARS-CoV / NL63-CoV 3CLpro:

[0048] Amino acid sequence of MERS-CoV 3CLpro: shown in SEQ ID NO:1.

[0049] SARS-CoV 3CLpro amino acid sequence: shown in SEQ ID NO:2.

[0050] NL63-CoV ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biomedicine, in particular to application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as a deubiquitinating enzyme and interferon inhibitor. The invention provides a novel deubiquitinating enzyme and interferon inhibitor; the new understanding of the functionality of human coronavirus proteinase can be expanded; theoretical basis can be provided for the research on coronavirus and host antiviral natural immunization and the research on antiviral drugs using viral proteinase as a target.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the use of MERS-CoV 3CLpro as a deubiquitinase and interferon inhibitor. Background technique [0002] Coronavirus (Coronavirus, CoV) is a typical member of the genus Coronavirus in the Coronaviridae family. It is a single-stranded positive-sense RNA virus that can infect vertebrates and humans and cause various diseases such as respiratory tract infection and gastroenteritis. The new human coronavirus-Middle East Resperatory Syndrome coronavirus (MERS-CoV) was first isolated from a 60-year-old Saudi patient who died of respiratory infection and renal failure at the end of 2012. According to the statistics of the World Health Organization, from September 2012 to April 2019, a total of 2,428 confirmed cases of MERS have been found in the world, of which 838 died, with a fatality rate as high as 35%, which seriously threatens human life and health (WHO website) . In recent year...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48G01N33/573
CPCC12N9/485C12Y304/19012G01N33/573G01N2333/948G01N2500/04G01N2500/10
Inventor 邢雅玲陈忠斌解晴陈晓娟
Owner ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com