87 results about "Genetic vaccine" patented technology

The invention concerns an expressible nucleic acid construct, which contains only the sequence information necessary for expressing a gene for RNA or protein synthesis. Expression constructs of this type can be used in gene therapy and genetic vaccination and avoid many of the risks associated with constructs today. The invention further concerns the possibility of improving the conveying of the construct into cells or tissue by covalent linkage of the construct, for example to particles or peptides.

Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

The present invention is directed to nucleic acid molecules encoding attenuated, non-functional virion infectivity factor (vif) proteins. The nucleic acid molecules of the invention are inserted into recombinant expression vectors and administered to mammals in order to induce a cellular and humoral immune response to the encoded protein product.

The invention provides a cationic lipid compound, its composition and application. In order to provide more options for the delivery of preparations such as nucleic acid drugs, gene vaccines, and small molecule drugs, the present invention proposes cationic lipid compounds represented by the general formula, or pharmaceutically acceptable salts thereof. The cationic lipid compound of the present invention can be used to deliver DNA, RNA or small molecule drugs, enriches the category of cationic lipid compounds, and has important significance for the development and application of nucleic acid preventive and therapeutic agents.

The invention concerns an expressible nucleic acid construct, which contains only the sequence information necessary for expressing a gene for RNA or protein synthesis. Expression constructs of this type can be used in gene therapy and genetic vaccination and avoid many of the risks associated with constructs today. The invention further concerns the possibility of improving the conveying of the construct into cells or tissue by covalent linkage of the construct, for example to particles of peptides.

The present invention provides methods for efficiently inducing anti-Aβ antibody and methods for preventing and treating Alzheimer's disease. The present inventors successfully induced anti-Aβ antibody in a highly efficient manner by administering an RNA viral vector that expresses a fusion protein between an AB5 toxin B subunit and an Aβ antigen peptide. Administration of the vector resulted in a significant increase of anti-Aβ antibody in plasma, and decrease in the Aβ level in brain tissues and decrease in the anti-Aβ antibody-positive area. The present invention enables more efficient vaccine gene therapy for preventing and treating Alzheimer's disease.

The present invention relates to genetic vaccines for stimulating cellular and humoral immune responses in humans and other hosts, and, in particular, relates to recombinant viruses that express heterologous antigens of pathogenic viruses, in single dose form.

The invention discloses an HPV(human papillomavirus) DNA (deoxyribonucleic acid) genetic typing method and a kit thereof. The method disclosed by the invention can be used for typing DNA of 46 types of HPVs, is simple to operate, is economical and practical and fast and efficient, and is combined with chip capillary electrophoresis testing to analyze the HPV DNA type with high throughput. When applied in the medical domain, the HPV / DNA genetic typing method is combined with the existing lamina ThinPrep cytology morphology diagnosis and is benefit to the early-stage prevention, prognosis and research of target gene vaccines for cervical cancer; and the method can be used for detecting multiple types and finding unknown types, thereby being especially suitable for research of pathogenesis and epidemiology of HPV-related diseases, such as hypopharyngeal carcinoma and head and neck cancer.

The invention belongs to the field of gene therapy and aims at providing a novel targeted gene vaccine for treating HBV (Hepatitis B Virus)-relevant liver cancer and application of the novel targeted gene vaccine in preparation of a targeted gene immunotherapeutic drug for the HBV-relevant liver cancer. The main active component of the liver caner-resistant vaccine disclosed by the invention is a recombinant vector of a gene coded with an HBx protein and a gene for coding a human IL-12 protein; and the recombinant vector can be used for simultaneously expressing the HBx protein and the human IL-12 protein in a eukaryotic cell. Provided by the experiment, a recombinant adenovirus vaccine disclosed by the invention can be used for selectively killing HBx-electropositive liver cancer cell and expresses a favorable liver cancer-resisting effect. The recombinant adenovirus vaccine provides a new selection for targeted gene immunization therapy of the HBV-relevant liver cancer and has a favorable application prospect.

The present invention provides genetic vaccines and methods for enhancing the immunity of a host, such as a human, against one or more pathogens. In one embodiment, a recombinant benign virus is provided as a genetic vaccine. The recombinant virus contains: an antigenic sequence heterologous to the recombinant virus, which encodes a viral antigen from the pathogenic virus. When the host is infected by the recombinant virus, the expression of the viral antigen causes the viral antigen to be directed to and in the host. an immune response in cells expressing the viral antigen; and an immunostimulator sequence heterologous to said recombinant virus, said immunostimulator sequence encoding an immune stimulator whose expression in the host increases the viral The immunogenicity of the antigen. The recombinant virus is replication incompetent and does not cause malignancies in the host that are naturally associated with the pathogenic virus. The genetic vaccine can be used to confer immunity to the host against many pathogens such as HIV, Ebola virus, hepatitis B virus, hepatitis C virus, influenza virus, pathogenic bacteria and parasites.

The invention discloses a multi-T cell epitope tuberculosis gene vaccine with HSP65 as an epitope scaffold. The full-length gene of HSP65 protein in which 5 T cell epitope polypeptide genes originated from mycobacterium tuberculosis antigen are embedded is inserted into a vector. A preparation method for the vaccine comprises a step of inserting the 5 T cell epitope genes originated from mycobacterium tuberculosis antigen into the HSP65 full-length gene, wherein the 5 T cell epitope genes comprise EAST-61-60, Ag85B721-765, MTB10.47-33, PPE25721-765 and PE1910-54. It is proved that the tuberculosis gene vaccine can induce response of serum IgG and lung SIgA to the HSP65 vector and induce cellular immunologic response of specific strong secretion of high-level IFN gamma to multiple tuberculosis antigen epitopes through intranasal immunization of mice with a nanometer granular compound formed by the gene vaccine and deacetylated chitosan, so the tuberculosis gene vaccine is a potential vaccine for prevention and treatment of tuberculosis.

Described are a method and a composition for transfecting monocytes, as well as use of the same for therapeutic purposes. The composition is composed of a nucleic acid component, a lysosome evading component and a digestible particle that can be phagocytized. Preferably, the monocyte is a macrophage and the digestible particle is from a natural source, such as from a microbial source. More preferably, the digestible particle is a yeast cell wall particle such as zymosan. The composition itself, or cells pretreated with the composition, are useful in all gene medicine applications, such as gene therapy, gene vaccination, cancer treatment as well as immunomodulation and tissue repair.