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Gene segments for coding alpha, beta or gamma interferons of pigs and application thereof

A technology of gene fragment and alpha interferon, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of low antiviral activity and low expression of original gene codons, and achieve high expression and good antiviral effect. , the effect of high potency

Active Publication Date: 2010-09-01
SOUTH CHINA AGRI UNIV +1
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Problems solved by technology

[0011] But the above technologies all have the defects of low expression level of original gene codon and low antiviral activity

Method used

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  • Gene segments for coding alpha, beta or gamma interferons of pigs and application thereof
  • Gene segments for coding alpha, beta or gamma interferons of pigs and application thereof
  • Gene segments for coding alpha, beta or gamma interferons of pigs and application thereof

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Embodiment Construction

[0025] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0026] (1) Genetic modification

[0027] Using DNASTAR (Version 5.0) gene analysis software, refer to the nucleotide sequence of porcine interferon α gene mature peptide (AY331298), porcine interferon β gene mature peptide nucleotide sequence (S41178) and porcine interferon γ gene published on NCBI GenBank Gene mature peptide sequence (EU249804), referring to the codon usage analysis of Pichia pastoris such as Zhao Xiang and the Pichia yeast codon table of TaKaRa company, etc., according to the amino acid sequence of the sequence, the expressed protein does not change, and at the same time according to Based on the partial tropism of codons in the expression system of Pic...

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Abstract

The invention relates to gene segments for coding alpha, beta or gamma interferons of pigs and an application thereof. According to the preference of codons of a Pichia stipitis expression system, high-expression codons are selected to renewedly design and synthesize new mature peptide nucleotide sequences of the alpha, beta and gamma interferon of the pigs, and recombinant yeast expression plasmids of pPICZalphaC-IFN-alpha, pPICZalphaC-IFN-beta and pPICZaplhaC-IFN-gamma are successfully constructed, which not only correctly expresses the alpha, beta and gamma interferons of the pigs in Pichia stipitis, but also enables the alpha, beta and gamma interferons of the pigs to be easier to express in the Pichia stipitis with higher expression level, and lays a foundation for the large-scale production and fermentation of the gene engineering.

Description

technical field [0001] The present invention relates to a gene fragment encoding porcine α, β or γ interferon and its application. Background technique [0002] In recent years, porcine viral infectious diseases have become increasingly rampant, which has seriously threatened the sustainable development of animal husbandry in my country. Immunosuppressive diseases and rapid mutation of viral antigens often lead to vaccine immunization failure; coupled with the continuous emergence of new viral diseases, there is no successful vaccination prevention yet. Vaccines, on the other hand, only prevent disease, and treatment also depends on the use of antibiotics. However, the emergence of some drug-resistant strains poses a great threat to human food and health. Some countries have explicitly banned the application of certain antibiotics and antibacterial agents in aquaculture production. Therefore, there is an urgent need for a new method that is both effective and environmental...

Claims

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Application Information

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IPC IPC(8): C12N15/21C12N15/22C12N15/24C12R1/84
Inventor 罗满林陈瑞爱刘健张欣唐明森
Owner SOUTH CHINA AGRI UNIV
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