Natural anti- virus and composition comprising thereof
A composition and technology of antiviral agent, applied in antiviral agent, botanical equipment and method, medical raw materials derived from virus/bacteriophage, etc., can solve problems such as destroying chicken body and immune system
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[0033] Preparation of Kimchi Lactic Acid Bacteria Culture Solution
[0034] Among the kimchi lactic acid bacteria, Leuconostoc kimchi (KCTC2386), Leuconostoc enterica subsp. enterica (KCTC3 530), Leuconostoc citreum (KCTC3 524), Leuconostoc lactis (KCTC3528), Lactobacillus plantarum (KCCM11322), and Weissella koreansis (KCTC3746) was used to prepare the culture solution.
[0035] First, 100 ml of MRS (Difco) broth was autoclaved at 121° C. for 15 minutes, and Kimchi lactic acid bacteria were inoculated into the broth, and shake cultured at 30° C. for 16 hours.
[0036] After the obtained culture was centrifuged at 5000×g for 3 min, the supernatant was filtered through a membrane filter having a pore size of 0.22 to obtain a sterile solution.
[0037] Subsequently, this sterile solution was passed through a membrane with a cut-off of 10,000 Da. The resulting filtrate was concentrated using a membrane with a cut-off of 1,000 Da. The concentrate thus obtained was filtered agai...
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