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Antiviral compound and its preparation method and application

A compound, influenza virus technology, applied in the field of biomedicine, can solve the problems of slow development of antiviral drugs, damage to host cells or organisms, etc., and achieve the effect of good antiviral activity

Active Publication Date: 2019-03-08
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the virus enters the body, it needs to replicate and reproduce in the host cell. Therefore, drugs that can inhibit virus reproduction have different degrees of damage to the host cell or the body, making the development of antiviral drugs has been relatively slow.

Method used

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  • Antiviral compound and its preparation method and application
  • Antiviral compound and its preparation method and application
  • Antiviral compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Preparation and identification of anti-influenza virus compounds.

[0061] 1. Preparation of anti-influenza virus compounds by fermentation

[0062] 1. Seed cultivation

[0063] (1) The slant medium was sterilized at 121° C. for 20 minutes to make a slant, and cultured at a constant temperature of 28° C. for 3 days. When the surface moisture is slightly dry and no bacteria grow, the spores of Mucor roxianus CGMCC 3.3447 are inoculated on the slant medium, and cultivated at 28°C for 3 days. It can be collected and used when it is infected with bacteria, and the slant bacteria can be obtained.

[0064] The above-mentioned slant medium is composed of the following components: potatoes, glucose, agar and water;

[0065] The concentration of each component in the slant medium is (g / L): potato (peeled, cut into small pieces and boiled for 20min, gauze filter) 200g / L, glucose 20g / L, agar 20g / L, the slant The pH of the culture medium is the natural pH, and the so...

Embodiment 2

[0088] The anti-influenza virus assay of embodiment 2, compound 1 and 2

[0089] 1. Cytotoxicity experiment

[0090] The 293T-IAV-Luc cells were passaged to a 96-well plate, and the number of cells per well was 10,000; after 14 hours of passage, the medium was discarded and the compound after serial dilution was added (the concentration was 40 μM, and two-fold serial dilution was performed 5 times, and the concentrations were 20 μM, 10μM, 5μM, 2.5μM L, 1.25μM); discard the medium after 48h of culture, add MTT medium with a final concentration of 0.5mg / ml, and incubate at 37°C for 4h; discard the culture medium, add 100μl DMSO to each well to avoid Lyse on an optical shaker for 10 min, and detect the absorbance at 570 nm with a microplate reader. The regression curve was calculated according to the obtained results, and the CC50 value was calculated.

[0091] 2. Determination of anti-influenza virus activity

[0092] Spread the 293T-IAV-Luc cells grown on a 10cm cell culture...

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Abstract

The invention provides an antiviral compound and a preparation method and application thereof. A structural formula of the compound is shown as a formula I or a formula II. The compound is prepared through the following method that Mucor roxianus CGMCC 3.3447 is utilized as a converting bacterial strain to perform microbial conversion on cryptotanshinone to obtain the compound. Pharmacological experiments show that the compound can inhibit growth of A / H1N1 influenza virus. Therefore, the compound can be applied to researching antiviral active lead compound or preparing antiviral medicine. The formula I and the formula II are shown in the specification.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an antiviral compound and its preparation method and application. Background technique [0002] Viruses are pathogens that seriously endanger human health, and more than half of infectious diseases are caused by viruses. In recent years, the worldwide spread of highly pathogenic and highly contagious viruses has drawn unprecedented attention from people around the world to viral diseases. Influenza A virus is a type A influenza virus, for example, it carries the H1N1 subtype swine influenza virus strain, which contains the ribonucleic acid gene fragments of the three influenza viruses of bird flu, swine flu and human flu, as well as Asian swine flu and African swine flu Influenza virus characteristics, is a human susceptible virus. After the virus enters the body, it needs to replicate and reproduce in the host cell. Therefore, drugs that can inhibit virus reproduction ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/04C12P17/18A61K31/34A61P31/16C12R1/785
CPCC07D493/04C12P17/18
Inventor 张立新刘雪婷何文妮李耀
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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