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Cadmium-resistant bacteria and method for inhibiting heavy metal cadmium absorption of paddy rice

A technology of bacterial inhibition and heavy metals, applied in the field of agriculture and environmental pollution control, can solve the problems of soil structure damage, content reduction, complex equipment, etc., achieve strong repair effect, reduce cadmium content of brown rice, and seedling growth without inhibition

Inactive Publication Date: 2014-07-30
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many remediation methods for soil heavy metal pollution. The commonly used physical and physicochemical remediation methods are generally expensive in investment, complicated in equipment and easy to cause damage to soil structure. However, some microorganisms have high resistance to heavy metals and strong adsorption capacity. Using these microorganisms to absorb and fix farmland Heavy metals in the soil can inhibit the absorption of heavy metals by crops, reduce their content in crops, and ensure the safety of agricultural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Screening of bacterial strain TCd-1

[0018] Dissolve 5 g of rice rhizosphere soil in 50 mL of sterile water, and put 150 μL of the solution in sterilized beef extract peptone liquid medium (medium: beef extract 3 g L -1 , peptone 10 g·L -1 , NaCl 5 g·L -1 , agar 20 g·L -1 , pH 7.3), shake on a shaker at 150 rpm at 37°C for 12 h. Then draw 0.1 mL from it and spread it on the -1 Five beef extract peptone solid medium plates with different cadmium concentrations were placed on each group with three replicates, and they were cultured upside down in a 37 °C incubator for 24 h. According to the growth of bacteria, take 300 mg·L -1 Streak culture was carried out on the plate medium with cadmium concentration, and the operation was repeated more than 3 times to observe the growth of the colony TCd-1, and the slant was stored in a refrigerator at 4 °C until use.

Embodiment 2

[0019] Example 2: Cadmium uptake properties of bacterial strain TCd-1

[0020] Under sterile conditions, the strain TCd-1 preserved in Example 1 was transferred to beef extract peptone liquid medium, and cultured with shaking at 150 rpm at 37°C for 12 hours was used as the seed liquid. Draw 1 mL of TCd-1 seed solution into 100 mL and the concentration of cadmium is 100 mg·kg -1 The beef extract peptone liquid medium was cultured with shaking at 37 °C for 24 h. Take 10 mL of the culture solution, centrifuge at 10000 rpm for 10 min, rinse the cell pellet with sterile water for 3 times, dry and weigh it, and use it to determine the cadmium content of the cell. After the same treatment as above, the bacterial cell pellet was suspended in 10 mL of sterile water, ultrasonically crushed for 140 s, and centrifuged at 12,000 rpm for 10 min to obtain the cell wall pellet, which was used for the determination of cadmium content in the bacterial cell wall, and the supernatant was used ...

Embodiment 3

[0023] Example 3: Restoration of rice growth under cadmium stress by bacterial strain TCd-1

[0024] The germinated rice seeds were taken and evenly implanted with 5 mg·L -1 CdCl 2 10 seeds were implanted in each petri dish, and the agarose medium implanted with no cadmium treatment was used as a control, and 5ml of distilled water (treatment Ⅰ) and bacterial solution of strain TCd-1 (treatment Ⅱ) Two treatments, each treatment repeated 5 times. Each treatment was placed in a greenhouse at 25°C for light cultivation. Water was supplemented quantitatively every day, and rice biomass was measured after 7 days. The results showed that under cadmium stress, the biomass of rice treated with TCd-1 bacterial solution was similar to that of the control. The plant height and fresh weight of rice treated with liquid solution increased by 38.4% and 38.1%, respectively, and the restoration effect was extremely significant. (As shown in table 2)

[0025] Table 2 Restoration effe...

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PUM

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Abstract

The invention relates to cadmium-resistant bacteria and a method for inhibiting heavy metal cadmium absorption of paddy rice, and belongs to the technical field of agricultural and environmental pollution treatment. The cadmium-resistant bacteria are (Pseudomonas sp.) TCd-1, is preserved in the China center for type culture collection of Wuhan university on December 11, 2013 and has the accession number of CCTCC M2013649. An identification result shows that the bacterial strain TCd-1 is Pseudomonas sp., has resistance to a plurality of heavy metals, and has cadmium, lead and nickel resistance concentrations of 900mg.L<-1>, 800mg.L<-1> and 600mg.L<-1>. A ratio of the number ratio active live bacteria in the bacterial liquid is 1.85-1.87*10<9> per milliliter. Through use of 2mL of the bacterial liquid in each kg of heavy metal cadmium-polluted wet soil, cadmium-caused paddy rice growth inhibition is overcome, cadmium content of all parts of the paddy rice is effectively reduced and especially, cadmium content of brown rice is reduced.

Description

technical field [0001] The invention relates to a cadmium-tolerant bacterium and a method for inhibiting rice from absorbing heavy metal cadmium, and belongs to the technical field of agriculture and environmental pollution control. technical background [0002] Cadmium is a highly toxic heavy metal element. Cadmium in the soil is easily enriched by crops and endangers human health through the food chain. At present, the area of ​​cultivated land polluted by toxic heavy metals in my country has reached 20 million hm 2 , accounting for about 1 / 5 of the total cultivated land area. Therefore, the goal of controlling heavy metal pollution in farmland is not only to suppress the toxicity of heavy metals to crops and increase their yield, but more importantly, to reduce the absorption of heavy metals by crops and inhibit their entry into the food chain. There are many remediation methods for soil heavy metal pollution. Currently, the commonly used physical and physicochemical rem...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/38
Inventor 林瑞余肖清铁周丽英谢惠玲李艺林文雄
Owner FUJIAN AGRI & FORESTRY UNIV
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