Inocula producing cellulase and use thereof

Abstract

The invention discloses a microbial inoculum of cellulase production and the application thereof. The active ingredients of the microbial inoculum of the cellulase production comprise pichia stipitis 1413, pichia stipitis 1515 and pichia stipitis 2526; wherein, the pichia stipitis 1413 is recombinant bacterium obtained by introducing endoglucanase gene into the pichia stipitis, the pichia stipitis 1515 is recombinant bacterium obtained by introducing exoglucanase gene into the pichia stipitis, and the pichia stipitis 2526 is recombinant bacterium obtained by introducing beta-glucosidase gene into the pichia stipitis. The cellulose can be produced by fermenting the microbial inoculum of cellulase production.

Description

technical field [0001] The invention relates to a cellulase-producing bacterial agent and its application. Background technique [0002] The traditional cellulase production method is mainly to screen excellent enzyme-producing strains in nature, and then obtain enzyme-producing strains for industrialized large-scale production through certain mutagenesis methods. However, the degradation of cellulose in the natural environment is accomplished by a variety of microorganisms. These microorganisms coexist with each other to form a micro-ecological system that degrades cellulose. In a specific ecosystem, microorganisms cooperate with each other and restrict each other, so that the ratio of the enzymes that produce cellulose is in an optimal state for degrading cellulose, and once the microorganisms that produce cellulase from If it is separated from its niche, it will destroy this optimal ratio. When screening excellent strains, it is undoubtedly separated from its ecological...

AI Technical Summary

Problems solved by technology

The former method needs to produce three kinds of enzymes separately, the whole fermentation period is long, and the requirements for equipment and floor space are relatively high.

However, the second method expresses three different enzyme proteins in one Pichia pastoris at the same time, and its expression level and yield cannot be guaranteed. It has only theoretical research value but no practical application significance.

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