Cellulase-producing fungus agent and application thereof

Abstract

The invention discloses a cellulase-producing fungus agent and application thereof. The cellulase-producing fungus agent contains the active components of pichia pastoris 1413, pichia pastoris 1515 and pichia pastoris 2526; the pichia pastoris 1413 is a recombinant bacterium which is obtained by introducing an endoglucanase gene into the pichia pastoris, the pichia pastoris 1515 is a recombinant bacterium which is obtained by introducing an exoglucanase gene into the pichia pastoris, and the pichia pastoris 2526 is a recombinant bacterium which is obtained by introducing a beta-glucosidase gene into the pichia pastoris. Cellulase can be produced by fermenting the cellulase-producing fungus agent.

Description

technical field [0001] The invention relates to a cellulase-producing bacterial agent and its application. Background technique [0002] The traditional cellulase production method is mainly to screen excellent enzyme-producing strains in nature, and then obtain enzyme-producing strains for industrialized large-scale production through certain mutagenesis methods. However, the degradation of cellulose in the natural environment is accomplished by a variety of microorganisms. These microorganisms coexist with each other to form a micro-ecological system that degrades cellulose. In a specific ecosystem, microorganisms cooperate with each other and restrict each other, so that the ratio of the enzymes that produce cellulose is in an optimal state for degrading cellulose, and once the microorganisms that produce cellulase from If it is separated from its niche, it will destroy this optimal ratio. When screening excellent strains, it is undoubtedly separated from its ecological...

AI Technical Summary

Problems solved by technology

The former method needs to produce three kinds of enzymes separately, the whole fermentation period is long, and the requirements for equipment and floor space are relatively high.

However, the second method expresses three different enzyme proteins in one Pichia pastoris at the same time, and its expression level and yield cannot be guaranteed. It has only theoretical research value but no practical application significance.