Method for promoting target rhizobium to migrate and colonize in alfalfa plant

Abstract

The invention discloses a method for promoting a target rhizobium to migrate and colonize in an alfalfa plant. An endogenous fluorescence labeling rhizobium R. GN5f liquid with cyan fluorescent protein (CFP) characteristics is obtained through a tri-parent conjugation method, natural plant source bacteriostatic agent matrine is added, and the endogenous fluorescence labeling rhizobium R. GN5f liquid and the matrine are mixed and inoculated to the root of gannong No.5 alfalfa in the reproductive growth stage. The method for promoting the target rhizobium to migrate and colonize in the alfalfa plant is advantaged in that (1), the target rhizobium R. GN5f is obtained through the tri-parent conjugation method, is good in genetic stability and convenient to use, and can be obtained easily; (2) the matrine restrains air source and soil source microorganisms, and does not restrain rhizobium, and promotes the migration and colonization of the rhizobium in the alfalfa, and is low in price, and is innocuous and unpoisonous to the environment; (3) the fluorescence labeling rhizobium liquid containing the matrine can be obtained easily, the inoculation operation is simple, short in consuming time, and inoculation can be carried out at any moment in the growth period; and (4) by adding the matrine, the migration and colonization of the target rhizobium are promoted, and the foundation is laid to obtain seeds with a lot of target rhizobia.

Description

technical field [0001] The invention relates to a method for promoting the migration and colonization of target rhizobia in alfalfa plants. Background technique [0002] The symbiotic nitrogen fixation between rhizobia and leguminous plants not only has huge economic and social benefits, but also has become a common agricultural measure in agricultural production to artificially inoculate rhizobia to promote crop growth and increase seed yield. However, the competitive nodulation ability of inoculated rhizobia can be affected by plant species, rhizobia species, special external natural environment, inoculation method, competition with indigenous rhizobia, and various biotic and abiotic stresses, making some rhizobia It is difficult to efficiently carry out nodulation and nitrogen fixation, and it is difficult to achieve the desired effect of increasing production. Previous studies have found that endogenous rhizobia carried by alfalfa seeds have obvious advantages in nodula...

AI Technical Summary

Problems solved by technology

This shows that the method of screening the target rhizobia in alfalfa in the vegetative growth period has certain guiding significance for the introduction of the target rhizobia, but whether the screening inoculation method is also beneficial to the alfalfa in the reproductive growth period needs to be further verified

For the example of alfalfa-rhizobium combination breeding, only preliminary research reports on the vegetative growth period have been done. At present, there are few studies on this breeding method at home and abroad.

The research on using the migration channel of rhizobia to change the ratio of endogenous rhizobia in seeds is still in the preliminary stage, and how to increase the number of target rhizobia in seeds and how to accurately and efficiently combine target rhizobia with improved alfalfa varieties is still blank

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