Rapid detection method for content of aflatoxin in brown rice based on FT-NIR technology

Abstract

The invention provides a rapid detection method for the content of aflatoxin in brown rice based on FT-NIR technology. The method comprises the following steps: step 1, sample preparation: a step of collecting brown rice sample with different Aspergillus flavus invasion degrees, crushing the brown rice samples to obtain sample powders and refrigerating the sample powders for detection; step 2, spectral detection: a step of collecting diffuse reflection spectral information of the sample powders by using a Fourier transform near-infrared spectrometer and determining the contents of aflatoxin B1, B2, G1 and G2 and a total amount thereof in the sample powders by using a multifunctional column purification-high performance liquid chromatography-fluorescence method; step 3, spectral pretreatment: a step of pretreating original spectral information of the sample powders obtained in the previous steps so as to eliminate interference; step 4, quantitative forecasting and analysis: a step of analyzing the pretreated spectral information by using a stepwise multivariate linear regression method (SMLR) and establishing a stoichiometrical model of the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof in the sample powders and the spectral information of the samples; and step 5, rapid determination: a step of outputting the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof by using the established model on the basis of the spectral information of to-be-detected brown rice.

Description

technical field [0001] The invention relates to the technical field of grain quality detection, in particular to a method based on FT-NIR technology to realize simultaneous detection of aflatoxin B in brown rice 1 , B 2 , G 1 , G 2 and a rapid detection method for its total level. Background technique [0002] Grains are rich in carbohydrates, fats, proteins and inorganic salt nutrients, and are important strategic materials related to the national economy and people's livelihood. However, under suitable moisture and temperature conditions, grains are extremely prone to mold and deterioration. According to reports, the post-harvest grain loss caused by mildew in my country is as high as 21 million tons each year, accounting for 4.2% of the total grain output in the country, which is more than four times the annual increase in grain output. [0003] There are about 200 kinds of common molds in grains, and there are more than 300 kinds of toxins produced by mold metabolis...

AI Technical Summary

Problems solved by technology

However, due to the loss of the protective effect of rice husk, brown rice is more susceptible to fungal contamination during storage and transportation and produces aflatoxin, which seriously threatens human health.

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