HBV detection method and kit, and application thereof
A detection method and kit technology, applied in the field of bacterial detection, can solve problems such as interference with HBVPgRNA concentration, inaccurate results, and quantitative contamination, and achieve the effects of good specificity, high sensitivity, and pollution prevention
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Embodiment 2
[0047] Qualitative detection of HBV virus
[0048] Present embodiment 2 provides a kind of qualitative detection method of HBV virus:
[0049] 1. PCR amplification reaction of HBV virus
[0050] 1) Reagents and components used in the PCR amplification reaction of HBV viral DNA
[0051] (1) HBV virus primer probe mixture:
[0052] Specific primer sequence F1 and HBV PgRNA specific probe RT-FP were designed for HBV PgRNA, F1 and RT-FP were compared with NCBI, especially 6 B types, 7 C types and 7 D types were selected The results of the Blast comparison are shown in 1a and 1b, and the results show that the matching results are 100% consistent, and the specificity of the primer design is high.
[0053] For the RT-R1 (R2) primer, the 3' end of the primer is 13 poly-T, and at the same time, in order to ensure the specificity, VN is introduced into the base sequence (wherein V represents the base A C G 3 kinds of bases, and N represents the base A C T G four bases), a non-human ...
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