Raman spectrum based detection method for oxidative rancidity of ganoderma lucidum spores oil

Abstract

The invention provides a Raman spectrum based detection method for oxidative rancidity of ganoderma lucidum spores oil. Acid value and peroxidation value of the ganoderma lucidum spores oil are taken as evaluation indexes of the oxidative rancidity degree of the ganoderma lucidum spores oil. The detection method includes taking Raman peak integral strength of 1655cm-1 in the Raman spectrum of a standard sample as a vertical coordinate and taking the acid value or the peroxidation value as a horizontal coordinate to create standard acid value and peroxidation value evaluation drawings of the ganoderma lucidum spores oil; detecting the Raman spectrum of a tested sample, and comparing a result of the Raman peak integral strength of 1655cm-1 with the standard acid value and peroxidation value evaluation drawings to acquire the acid value and the peroxidation value of the tested sample to further acquire an oxidative rancidity degree evaluation result of the tested sample of the ganoderma lucidum spores oil. The Raman spectrum based detection method has the advantages of short detection time, low sample consumption, simplicity and convenience in operation and on-site quick detection, thereby having a broad practical application prospect.

Description

(1) Technical field [0001] The invention relates to a method for detecting oxidative rancidity of ganoderma lucidum spore oil, in particular to a method for rapidly detecting the degree of oxidative rancidity of ganoderma lucidum spore oil based on Raman spectroscopy. (2) Background technology [0002] Ganoderma lucidum, also known as Ganoderma lucidum, is the whole plant of Chizhi or Zizhi, a polyporaceae plant. It has been praised as a top-grade Chinese medicine since ancient times, and it has long been recorded in "Shen Nong's Materia Medica" and other traditional Chinese medicine classics. Ganoderma lucidum spores are the sexual reproductive cells of Ganoderma lucidum, generally light brown to yellowish brown, oval in shape, generally in the size of (length×width)(8.5~11.2)μm×(5.2~6.9)μm, containing Ganoderma lucidum spore oil . Ganoderma lucidum spore oil concentrates the essence of active ingredients of Ganoderma lucidum, rich in triterpenoid ganoderma acid, polysacch...

AI Technical Summary

Problems solved by technology

The domestic methods for determining the acid value and peroxide value of Ganoderma lucidum spore oil are mainly titration methods, including standard acid-base titration and redox titration, but the titration method has the following defects: as the operation is complicated, it needs professionals to analyze in the laboratory; It takes a long time, and the detection takes several hours to complete; it is not portable, and it is difficult to detect outdoors; the sensitivity is not high enough, the sample consumption is large (5-10g), and the detection cost is high

These methods have certain advantages compared with traditional methods, but they all have certain limitations when testing, especially for more expensive substances such as Ganoderma lucidum spore oil

For example, high-performance liquid chromatography (HPLC), compared with traditional methods, has the advantages of high speed, no sample damage, and easy recovery, but it is not easy to carry and difficult to detect outdoors. Compared with Raman spectroscopy, its sensitivity is still not as good as Raman Spectrum, time-consuming

Chromatography (GC) detection, although its resolution efficiency is high, quantitative analysis is simple, but its qualitative ability is poor

Mass spectrometry (MS), corresponding to chromatography, has strong qualitative ability and high sensitivity, but it requires pure samples during the detection process, and the quantitative analysis is more complicated.

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