Method of detecting biological molecules by nano enzyme immune sandwich novel technology
A technology of biomolecules and nanozymes, which is applied in the field of nanomaterials and biomedical nanotechnology, can solve the problems of complicated steps, difficult to realize automatic operation, and long time-consuming
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Embodiment 1
[0051] Embodiment one detects ricin
[0052] In this embodiment, the method for detecting biomolecules by the nano-enzyme immune sandwich technology is as follows:
[0053] (1) The monoclonal antibody 6A6 of ricin was coupled to silicon dioxide-embedded iron ferric oxide magnetic particles with a particle size of 1 micron by EDC-NHS activated carboxyl method to make a capture probe;
[0054] The specific steps of the coupling are as follows: Weigh 1 mg of ferroferric oxide magnetic particles modified by silica with a particle size of 1 micron, add N-hydroxysuccinimide NHS solution with a concentration of 50 mg / mL and a concentration of 50 mg / mL 50 μL of each mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride EDC solution, incubate at room temperature for 30 minutes, wash with deionized water to remove excess NHS / EDC; add 1 mL , sodium acetate solution with a pH of 6.0 and a concentration of 50mmol / L, and 100 μg of anti-ricin monoclonal antibody 6A6, mixed well,...
Embodiment 2
[0063] Example 2 Detection of tumor marker alpha-fetoprotein AFP
[0064] In this embodiment, the method for detecting biomolecules by the nano-enzyme immune sandwich technology is as follows:
[0065] (1) The monoclonal antibody Ab-1 of alpha-fetoprotein AFP is coupled to dextran-modified particle diameters on iron ferric oxide magnetic particles of 2 microns by periodic acid oxidation method to make a capture probe;
[0066] The specific steps of coupling are as follows: Weigh 1 mg of dextran-modified ferroferric oxide magnetic particles with a particle size of 2 microns and dissolve them in 1 mL of freshly prepared NaIO with a concentration of 0.05 mol / L. 4 The solution was stirred at room temperature for 20 minutes in the dark; the supernatant was removed by magnetic adsorption, and washed three times with sodium acetate buffer solution with a concentration of 1 mmol / L and a pH of 4.4; salt buffer solution (such as buffer solution prepared with sodium carbonate and sodium...
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