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Beta 2-acceptor stimulant ELISA kit and usage method and application thereof

A technology of enzyme-linked immunosorbent reagents and receptor agonists, which is applied in the direction of biological testing, material inspection products, etc., can solve problems such as accelerated myocardial contraction, patient shock, bioelectrical disturbance, etc., and achieves wide application range, convenient use, and low cost Effect

Inactive Publication Date: 2013-08-07
天津康利缘生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2) Since it reduces the Mg that inhibits muscle excitability 2+ Concentration, lead to bioelectrical disorder, the patient's muscle excitability increased, causing muscle tremors, gastrocnemius muscle spasm, vomiting and diarrhea, and at the same time, myocardial contraction and heart rate increased, which could easily cause shock and death in patients with high blood pressure
[0089] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Beta 2-acceptor stimulant ELISA kit and usage method and application thereof
  • Beta 2-acceptor stimulant ELISA kit and usage method and application thereof
  • Beta 2-acceptor stimulant ELISA kit and usage method and application thereof

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Embodiment Construction

[0128] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0129] The kit of the present invention synthesizes an immunogen from salbutamol (SAL) and ovalbumin (OVA) by the mixed acid anhydride method, and prepares a polyclonal antibody by immunizing sheep with the coupled immunogen, and preliminarily purifies it by ammonium sulfate precipitation, and uses affinity chromatography The further purification is identified by ELISA method, and the enzyme-labeled antigen is formed by cross-linking horseradish peroxidase and albuterol hapten with sodium periodate. Using the obtained antibody and enzyme-labeled antigen to establish a direct competitive enzyme-linked immunosorbent assay (ELISA) for the detection of β 2- For receptor agonists, the minimum detection limit is 0.1 ng / mL.

[0130] The assay p...

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Abstract

The invention relates to a beta 2-acceptor stimulant ELISA kit, which comprises the following compositions in proportion: a coating plate, a calibrator, an enzyme labelling object operating fluid, a substrate liquid A, a substrate liquid B, a stopping solution, a condensed washing liquid and a condensed extract. The kit sensitivity is 0.1 ppb, and the precision is as follows: intra assay variation coefficient (CV%) is 5%, and inter assay variation coefficient (CV%) is 10%; the accuracy is shown by recovery rate, and the recovery rate is in the range of 80-110%. IC 50 range is between 0.3 and 0.5 ppb, and the linear correlation coefficient |r| is not less than 0.9900, and the product has the advantages of wide usage scope, convenient usage and accurate detection.

Description

technical field [0001] The invention belongs to the field of detection kits, and relates to a clenbuterol detection kit, especially a beta 2 -Receptor agonist enzyme-linked immunosorbent assay kit and its use method and application. Background technique [0002] In recent years, with the development of animal husbandry, the use of veterinary drugs has become increasingly widespread, and has gradually become one of the indispensable material bases. including beta 2 - Veterinary drugs including receptor agonists have indeed played a very significant role in promoting growth, improving feed conversion rate and utilization rate, and improving product quality. However, while promoting the development of animal husbandry, the use of veterinary drugs will undoubtedly lead to the retention or accumulation of the drug itself and its metabolites in the animal body, and enter the human body and the ecosystem in a residual manner, causing harm. Animal-derived food safety issues, espe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 钟凯
Owner 天津康利缘生物工程有限公司
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