71results about How to "Quantitative detection" patented technology

The invention provides a cTn I (cardiac troponin I) hypersensitive detection kit and a preparation method thereof. The kit comprises at least one strain of a first anti-cTn I antibody marked with a tracing marker and at least one strain of a second anti-cTn I antibody coated with magnetic micro-spheres, wherein a binding site between the first anti-cTn I antibody and cTn I is different from a binding site between the second anti-cTn I antibody and cTn I; and the kit can preferably further comprise a diluent, and by adopting the diluent, the non-specific binding in a detection process can be significantly reduced, and the detection accuracy and sensitivity can be further improved. The invention also provides a method for detecting cTn I by using the kit, and the method provided by the invention has very high sensitivity, can be used for sensitively and accurately detecting the cTn I content in a sample, and can provide more timely and reliable information for early diagnosis and treatment of AMI.

The invention provides a method and device for detecting hydrogen leakage of a vehicle-mounted hydrogen supply system and a fuel cell vehicle. The method comprises the steps: determining the current working condition of the fuel cell vehicle; determining a detection time period according to the current working condition; obtaining the hydrogen leakage quality of the vehicle-mounted hydrogen supplysystem in the detection time period; calculating the hydrogen leakage rate according to the hydrogen leakage quality and the detection time period; and when it is judged that the hydrogen leakage rate is larger than a preset value, determining that the hydrogen leakage occurs in the vehicle-mounted hydrogen supply system. According to the technical scheme provided by the invention, hydrogen leakage of the vehicle-mounted hydrogen supply system of the fuel cell vehicle can be quantitatively detected by calculating the hydrogen leakage rate, the accuracy and timeliness of hydrogen leakage detection are improved, and the problems of potential safety hazards and waste caused by hydrogen leakage are solved.

The invention provides a super-paramagnetic nanoparticle for capturing exosomes and a preparation method thereof as well as a specific exosome luminescence immune quantitative detection kit, and belongs to the technical field of exosome detection. The super-paramagnetic nanoparticle for capturing the exosomes, provided by the invention, comprises a super-paramagnetic nanoparticle base body and anexosome shared marker antibody coupled with the super-paramagnetic nanoparticle base body. The specific exosome luminescence immune quantitative detection kit provided by the invention comprises the super-paramagnetic nanoparticle for capturing the exosomes, an exosome specific marker antibody for luminescence labeling, confining liquid, a washing solution, a NaOH water solution, hydrogen peroxideand a calibrator, and can realize simple, rapid and quantitative detection of the specific exosomes; the specific exosome luminescence immune quantitative detection kit can be directly used for detecting a marker of the specific exosomes in blood serum, blood plasma, pleuroperitoneal fluid, urine and cerebrospinal fluid samples; the specific exosome luminescence immune quantitative detection kithas the advantages of high sensitivity, good stability, short detection time and simplicity in operation and is very applicable to clinical detection.

The invention provides a heart failure detection method based on an electrocardio-interval sequence normalized histogram and a device thereof. The detection method comprises the following steps: (1) electrocardiosignals of a testee are collected; (2) the analog-digital conversion is carried out on the electrocardiosignals, and electrocardio-waveform data are stored; (3) R wave peak value points of the electrocardiosignals are extracted to construct an electrocardio-interval sequence RR; (4) the effectiveness of the RR sequence is tested; (5) the RR electrocardio-interval sequence normalized histogram is constructed; (6) two specific indexes of center-edge rate (CER) and cumulative energy (CE) of the normalized histogram are calculated; (7) the heart failure course of the testee is evaluated according to the indexes of CER and CE. The detection device comprises an electrocardiosignal detection module, an analog-digital conversion device and a computer. Based on the two specific indexes of CER and CE of the electrocardio-interval sequence normalized histogram, the heart failure course of the testee is evaluated, the heart failure course of the testee can be detected noninvasively, conveniently and quantitatively, and the discrimination index is high.

A raman spectroscopy method of measuring melamine contents in dairy products having different matrixes. The method includes: (a) establishing a database of characteristic curves of dairy products having different matrixes; (b) taking several copies of the dairy products having one certain unknown matrix and adding melamine standard solutions having different concentrations therein, to obtain a series of dairy product samples in which the relative concentrations of the melamine are known; (c) performing raman spectrum testing analysis and obtaining corresponding characteristic peak intensities to obtain a slope of the characteristic curve showing variation of the characteristic peak intensities with the relative concentrations of the melamine; (d) searching the database of step (a) using the slope of the characteristic curve of the dairy product samples to find a matching characteristic curve, and (e) calculating concentration of melamine in the dairy products by using the matched characteristic curve and the characteristic peak intensity.

The invention discloses a mechanical sealing leakage detection system and method applied to long pipeline delivery oil pumps. The method includes acquiring leakage liquid quantity, when the detected the leaked liquid quantity meets the drop counting condition, acquiring a first flow signal of the leaked liquid quantity by the optical detection method; determining a first flow of the leaked liquid quantity according to the first flow signal; when the leaked liquid quantity cannot meet the drop counting condition, acquiring a second flow signal of the leaked liquid quantity by the optical detection method; determining a second flow of the leaked liquid quantity according to the second flow signal. Thus, mechanical sealing leakage can be detected online quantitatively, and the mechanical sealing leakage quantity can be detected accurately in real time.

The invention belongs to the field of preparation of nanomaterial and fluorescence probe and bacteriostasis, and relates to a preparation method of a molybdenum oxide quantum dot by a one-step method.The method includes steps of weighing fixed quantity of molybdenum oxide in peeling solvent (including but not limiting in dimethyl sulfoxide); stirring to obtain milky suspending solution; performing ultrasound treatment on the milky suspending solution for 2 hours under the condition of more than 280W power; adding a rotor in the suspending solution after ultrasound treatment; then stirring thesolution added with rotor by a magnetic stirrer at the speed of more than 250 r / m for more than 5 hours; finally, adding the stirred solution in a centrifugal pipe; centrifuging at the rotate speed of more than 10000 r / m for more than 5 minutes, and taking liquid supernatant after separation of solid and fluid, so as to obtain the molybdenum oxide quantum dot. The technique process is simple andlow-consumption, and able to reliably and stably prepare the molybdenum oxide quantum dot; the molybdenum oxide quantum dot can be used as a fluorescence probe and can detect ferric ion and pyrophosphate anion at fixed amount in real time.

The present invention relates to an immunochromatography time resolution fluorescence kit for synchronization detection of mixed pollution of fumonisin B1 and other four fungaltoxin and application thereof. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and a sample reaction bottle containing europium-labeled freeze-dried products of all monoclonal antibodies; the immunochromatography time resolution fluorescence test paper strip includes a PVC substrate, a water absorbent pad, a testing pad and a sample pad which are respectively pasted on one side of the PVC substrate from top to bottom, the testing pad is provided with a nitrocellulose membrane as a base pad, the nitrocellulose membrane is provided with a horizontal quality control line and four detection lines from top to bottom, the four detection lines are respectively coated with bovine serum albumin conjugates of all the fungaltoxin, and a fumonisin B1 monoclonal antibody is secreted by hybridoma cell line Fm7A11 with preservation number of CCTCCNO.C201636. The immunochromatography time resolution fluorescence kit can be used for synchronization detection of content of aflatoxin B1, ochratoxin A, zearalenone and the fumonisin B1, and has the characteristics of simple operation, rapidness and high sensitivity.

The invention relates to a rapid, simple, convenient and sensitive detection method for related impurities of glucosamine salt medicines, which is characterized in that the detection is performed according to high performance liquid chromatography in pharmacopoeia of the people's republic of China (Edition 2010). A chromatographic condition and system adaptability test comprises the steps of: adopting an amino column as a detection column; preparing a phosphate buffer solution, wherein 0.7 g of KH2PO4 is diluted to 1000 ml by adding water and the pH value is adjusted to be 7.5 by adding a stronger ammonia water solution; adopting the phosphate buffer solution and acetonitrile as a mobile phase, wherein the volume ratio of the phosphate buffer solution to the acetonitrile is 35:65; and detecting wavelength of 195 mm, the flow rate of 1.0 ml / min, the column temperature of 35 DEG C, and the theoretical plate number of not less than 800in the terms of glucosamine hydrochloride . The method overcomes the characteristics of poor sensitivity and poor specificity and the like in the detection by adopting a thin layer detection method in the prior art.

The invention discloses an N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof. The kit comprises: reagents I: a phosphate buffer solution, a preservative, Tween 20 and polyethylene glycol; reagents II: a phosphate buffer solution, disodium hydrogen phosphate, sodium dihydrogen phosphate of 2 to 5 g / L, sodium chloride, a preservative and sensitized latex particles of a goat anti-human N-terminal-front B type natriuretic peptide antibody; and a calibration product: a phosphate buffer solution, bovine serum albumin, sodium chloride, a preservative and N-terminal-front B type natriuretic peptide antigen. According to the method, the test sensitivity is improved by applying a latex immunoturbidimetric method, the sensitized latex particles suitable for detecting an N-terminal front B type natriuretic peptide precursor are found, and the detection sensitivity is improved; large-batch samples can be detected quantitatively and simultaneously; and the kit comprises the calibration products and the quantitative detection aim is fulfilled.

The invention relates to an amino-terminal pro-brain natriuretic peptide direct chemiluminiscence detection kit and a preparation method and application thereof. Particular, the kit provided by the invention comprises a first antibody coating magnetic microspheres and a second antibody marked with a tracing marker, wherein the first antibody and the second antibody are monoclonal antibodies of anti-amino-terminal pro-brain natriuretic peptide, and the specific binding sites of the amino-terminal pro-brain natriuretic peptide recognized by the first antibody and the second antibody are different. The kit can be used for determining whether a to-be-detected sample contains the amino-terminal pro-brain natriuretic peptide and / or the content of the amino-terminal pro-brain natriuretic peptide,can be used for accurately diagnosing the severity of heart failure as soon as possible, provides sufficient time for heart failure treatment, and provides sufficient support for prognosis detectionof heart failure patients.

The invention relates to a preparation method of an electrochemical sensor for detecting residual pesticides. A novel wire-mesh electrochemical sensor based on a graphene modified electrode is prepared by using a screen printing technology. The electrochemical sensor provided by the invention can realize simultaneous detection on isoproturon and carbendazim through simple sample dripping measurement; the electron transfer of isoproturon and carbendazim can be effectively enhanced so that isoproturon and carbendazim can be simply, rapidly and quantitatively detected. The electrochemical sensor provided by the invention realizes the rapid and simple simultaneous detection on isoproturon and carbendazim pesticides in environment water, soil and foods.

The invention relates to a vancomycin-resistant enterococcus multiplex fluorescence quantitative PCR (polymerase chain reaction) detection kit. The kit comprises a VanA specific primer, a VanB specific primer, a VanA fluorescent probe, a VanB fluorescent probe, a PCR buffer solution, a deoxynucleoside triphosphate mixture, a DNA (deoxyribonucleic acid) polymerase, a VanA gene standard product sequence and a VanB gene standard product sequence. The kit disclosed by the invention has the beneficial effects that due to the adoption of the PCR detection method, accurate, fast and quantitative detection can be realized.

The invention relates to an SARS-CoV-2 neutralization detection kit, which comprises a solid phase carrier, a screening protein, a competitive binding protein, alkaline phosphatase and an alkaline phosphatase luminescent substrate, wherein the screening protein comprises an RBD recombinant protein having an amino acid sequence represented by SEQ IDNO.1 or having at least 85% homology, and / or the fusion protein of the S protein and the N protein with the amino acid sequence as shown in SEQ IDNO.2 or having at least 85% of homology with the fusion protein. The kit developed by the invention can be used for efficiently, safely, quantitatively and accurately detecting the SARS-CoV-2 neutralization antibody, and large-batch rapid detection can be realized. And the RBD protein recombined by the eukaryotic expression system or the fusion protein of the S protein and the N protein is selected as the screening protein of the competitive reaction system, so that the sensitivity and the specificity of the detection kit can be further improved.

The invention discloses a rapid-forming automatic powder spreading quantity adjusting closed-loop control system. The system comprises a powder spreading time detection mechanism, a powder spreading controller, a computer, a servo motor, a servo driver and a zero-point travel switch; the servo driver is connected with the output end of the powder spreading controller; the servo motor is connected with the servo driver; the zero-point travel switch is mounted on a powder spreading platform and connected with the input end of the powder spreading controller; the powder spreading time detection mechanism comprises a fixed support, an upper powder falling pipe, a lower powder falling pipe and a photoelectric sensor; a transparent pipe is connected between the upper powder falling pipe and the lower powder falling pipe; the emission end and the receiving end of the photoelectric sensor are located on the two sides of the transparent pipe, respectively; the input end of the powder spreading controller is connected with a timer; the signal output end of the photoelectric sensor is connected with the signal input end of the timer. The invention also discloses a rapid-forming automatic powder spreading quantity adjusting closed-loop control method. The rapid-forming automatic powder spreading quantity adjusting closed-loop control system is capable of realizing high-precision control of the powder spreading quantity and improving the forming precision and quality of parts.

The invention belongs to the technical field of fluorescent probes, and provides a fluorescent probe for quantitatively detecting riboflavin on the basis of a fluorescence resonance energy transfer ratio, and a preparation method and an application for the fluorescent probe. The preparation method comprises the following steps: with glucose as a carbon source, ethylenediamine as a nitrogen source,concentrated phosphoric acid as a phosphorus source and concentrated hydrochloric acid as a chlorine source, preparing nitrogen-phosphorus-chlorine co-doped carbon quantum dots (NPCl-CQDs) through anacid-base neutralization reaction exothermic carbonization method, dissolving NPCl-CQDs powder into ultrapure water, carrying out centrifuging to remove insoluble substances, carrying out filtering to remove impurities, and carrying out freeze-drying so as to obtain the fluorescent probe, namely NPCl-CQDs solid powder. The linear relationship between a riboflavin concentration and an NPCl-CQDs fluorescence intensity is determined by using a ratio fluorescence detection method. The content of riboflavin in an actual sample is detected through a standard recovery experiment, and a standard recovery rate is calculated. The method provided by the invention is simple and convenient to operate, low in background interference, high in sensitivity, free of expensive instruments and equipment, lowin detection cost, capable of rapidly, efficiently and quantitatively detecting the content of the riboflavin in an actual sample in a ratio and good in reproducibility.

The invention relates to a light leak detection device. The light leak detection device comprises a clamp unit and a detection device body. The clamp unit comprises a base. A fixing block is arranged at one end of the base, and a fixing sliding block is arranged at the other end of the base. An electronic product to be detected is installed between the fixing block and the fixing sliding block. Light blocking foam is arranged on the portion, between the fixing sliding block and the fixing block, of the base. A groove is formed in the portion, between the light blocking foam and the fixing block, of the base, and the detection device body is arranged in the groove. The arranged light blocking foam has the effect of preventing the surface of a display screen from making contact with the base in the detection process to protect the surface of the display screen and also has the effect of forming a closed space together with the fixing block to guarantee a sufficient detection light source; a plurality of sensors are arranged, an MCU control unit sends data to an external PC for calculation, and therefore the detection process is quantified.

The invention, which relates to the technical field of vehicle braking performance detection, provides an apparatus for detecting a braking performance of a damaged vehicle. The apparatus comprises a loading rod, a braking force loader, a braking force sensor, a brake pedal force sensor installed at a pedal, a data acquisition card, and a computer. A flange connected with a wheel is arranged at one end of the loading rod and an adjusting block capable of adjusting a loading position is arranged at the other end. The braking force loader is arranged right below the adjusting block; and the braking force sensor is arranged between the braking force loader and the adjusting block. The braking force sensor and the brake pedal force sensor are respectively connected with the data acquisition card; and the data acquisition card is connected with the computer. With the apparatus, a braking performance of a vehicle that can not be driven due to damages caused by a traffic accident can be detected scientifically, accurately, and quantificationally. The provided apparatus has advantages of simple structure, small size, low cost, high portability, and good detection effect.

The invention discloses a method for rapidly detecting the content of B vitamins in pig intestinal contents / excrement. The method comprises the following steps: weighing cecum contents / excrement of pigs as a sample, adding a methanol / acetonitrile / water solution, and performing homogeneous extraction to obtain a sample solution; and performing UPLC-MS / MS-MRM detection analysis on the sample solution to finally obtain the content of eight B vitamins in the sample, wherein the eight B vitamins are vitamin B1, vitamin B2, nicotinic acid, nicotinamide, pyridoxal, pyridoxine, vitamin B9 and vitaminB12. According to the method, an MRM technology is utilized, an ultra-high performance liquid chromatography system and a mass spectrometer are combined, and the content of a plurality of B vitamins in the samples such as the pig intestinal contents and the excrement can be rapidly detected based on targeted metabonomics of an MRM method.

The invention belongs to the technical field of fluorescent probes, and provides a biomass carbon dot fluorescent probe for ratio quantitative detection of adriamycin as well as a preparation method and application of the biomass carbon dot fluorescent probe. The method comprises the following steps: with 150-mesh corncob powder as a carbon source, a nitrogen source, a sulfur source and a phosphorus source, heating and carbonizing the 150-mesh corncob powder in a high-pressure reaction kettle at 180 DEG C for 12 hours to prepare a biomass carbon dot solution; and conducting centrifuging to remove insoluble substances, and performing freeze-drying to obtain biomass carbon dot solid powder. According to the invention, a linear relation between the concentration of adriamycin and the fluorescence intensity of a biomass carbon dot are determined by utilizing a ratio fluorescence detection method; and the content of adriamycin in an actual urine sample and the adding standard recovery rate of adriamycin are detected by utilizing a constructed linear equation. The method is simple and convenient to operate, strong in anti-interference performance, free of expensive instruments and equipment and low in detection cost, has self-calibration performance, can rapidly, efficiently, sensitively and quantitatively detect adriamycin in an actual sample, and has good reproducibility.

The invention discloses an application of an acetylcholinesterase piezoelectric biosensor in detecting nerve bundle property. A choline esterase antibody fixed on a quartz crystal reacts to acetylcholinesterase contained in a nerve sample to be measured, the oscillation frequency of the quartz crystal changes in the reaction process, then the content of the acetylcholinesterase is measured and mirrored through a computer monitor system, the nerve bundle to be measured with high content is of a motion tract, and the nerve bundle to be measured with low content is of a sensory tract. The invention starts with the difference of the content of the acetylcholinesterase in the peripheral nerve motion tract and the sensory tract, can fast detect an acetylcholinesterase piezoelectric biosensor system, fast discriminate the peripheral nerve motion tract and the sensory tract, and realize the correct sewing of the motion tract to the motion tract, and the sensory tract to the sensory tract. The invention can be widely popularized in the further clinical application.

The invention belongs to the technical field of coating testing methods, and particularly relates to a ceramic plate binding force pull-off testing method. The method comprises the steps: cutting outa square block with a certain size from a ceramic copper-plated plate to obtain a ceramic copper-plated plate detection sample; brushing soldering paste on the copper layer on the other side of the ceramic copper-plated plate detection sample, and then pressing the ceramic copper-plated plate detection sample and a copper foil together; carrying out heating at a constant temperature of 240-255 DEGC, taking down the ceramic copper-plated plate detection sample after the soldering paste is molten, and cooling to prepare a detection sample A; placing the detection sample A on a tension platformof a stripping testing machine, and fixing the detection sample A; welding one end of a tension copper wire to the detection sample A, and fixing the other end of the tension copper wire to a stripping testing machine stretching device; and starting a tensile program of the stripping testing machine, and carrying out pull-off test on the film layer to obtain the binding force. The ceramic plate binding force pull-off test method can quantitatively and qualitatively detect the binding force of the ceramic copper-plated plate coating, and the detection method is simple and easy to implement.

The invention discloses a high-sensitivity mutation site detection system, a high-sensitivity mutation site detection method and application. The high-sensitivity mutation site detection method comprises the following steps of designing wild-type forward or reverse primers aiming at wild-type genes, mutant forward or reverse primers aiming at mutant genes, universal forward or reverse primers, mutant blockers and wild-type blockers; using a double-tube PCR system for PCR reaction; and performing quantitative PCR detection on a sample, wherein one tube comprises wild-type primers, mutant blockers and universal primers, and the other tube comprises mutant primers, wild blocks and universal primers. The high-sensitivity mutation site detection method provided by the invention has the advantages that by using a method of combining specific primers with specific blockers, the specificity and the sensitivity of the detection are improved. Through the standard product and standard curve drawing, the false positive and false negative results are well avoided. The capability of detecting gene mutation of ten thousandth or higher can be realized.

The invention relates to a high-sensitivity hydrogen sensor at a room temperature and a preparation method thereof. The hydrogen sensor comprises a substrate and a hydrogen sensitive layer in sequence, and the hydrogen sensitive layer is a palladium-doped tungsten trioxide film. The preparation method comprises the following steps: (1) a graphene / Si heterostructure Schottky junction is prepared; (2) a tungsten trioxide sol is prepared; and (3) spin coating of the obtained tungsten trioxide sol on the graphene / Si heterostructure Schottky junction is carried out to obtain a gasochromic WO3 / graphene / Si series sensor, that is, the high-sensitivity hydrogen sensor at the room temperature. In comparison with the prior art, visual detection can be carried out, the reaction response time is short,the sensitivity is high, the use is convenient, the operation is easy, and the purpose of accurately and timely detecting hydrogen leakage is thus achieved.

The invention provides a seawater organic phosphorus concentration detection device, comprising a data acquisition control unit, a storage unit, a network transmission unit and a host computer; the host computer sends a collection instruction to the data acquisition control unit through the network transmission unit, The data acquisition control unit collects organic phosphorus concentration data of seawater samples, and uploads to the host computer through the network transmission unit for calculation to obtain a concentration value, and the storage unit caches the collection instruction and the concentration data. The present invention simultaneously tests seawater containing organic phosphorus and seawater without organic phosphorus through the electrochemical enzyme sensor of four detection channels, combines FIFO high-speed buffer storage and Ethernet communication technology, and accurately and quickly measures seawater samples by standard addition method The concentration of organic phosphorus in the system realizes automatic control of the entire detection device and real-time and rapid reading of measurement data.

The invention provides a molecular marker for detecting DNA methylation of cow mastitis resistance and a primer pair of the molecular marker. According to an amplification primer pair and a sequencing primer pair designed for the molecular marker, the DNA methylation of cow mastitis resistance can be detected. The detecting method comprises the following steps: treating the genome DNA of a cow to be detected through bisulfite, performing PCR amplification on a TRAPPC9 gene by using the amplification primer pair, and sequencing by using the sequencing primer pair and a pyrophosphoric acid instrument, so as to obtain the methylation level of a TRAPPC9 gene promotor region of the cow to be detected. According to the invention, a theory basis and a hereditary basis are provided for molecular breeding of cows with mastitis resistance.

The invention belongs to the field of fluorescent probes, and relates to a fluorescent probe based on a fluorine-boron skeleton, in particular to a fluorine-boron skeleton-based fluorescent probe TQBF-NBD with large Stokes displacement, and a preparation method and application of the fluorescent probe TQBF-NBD. The fluorescent probe TQBF-NBD has a structural formula as shown in the specification.The molecule of the probe does not have fluorescence, and emits red and green fluorescence after reacting with biological mercaptan. The probe molecule provided by the invention not only can qualitatively analyze the biological mercaptan, but also can realize rapid and quantitative detection of the biological mercaptan, and has important application value in the fields of biochemistry and the like.

The invention discloses a composite modified electrode as well as a preparation method and application thereof. The gold nano-poly(3-aminophenylboronic acid) chemically modified electrode has the advantages of high sensitivity, high detection speed, no additional indicator and simple operation, and can realize rapid, accurate and quantitative detection of aluminum ions and xylitol. Detection results show that indexes such as a linear range, a detection limit and reproducibility all meet requirements, and the modified electrode can be successfully used for food detection and analysis and further used for evaluating food quality.