Application of acetylcholine esterase piezoelectricity biosensor in the detection of nerve tract property

Abstract

The invention discloses an application of an acetylcholinesterase piezoelectric biosensor in detecting nerve bundle property. A choline esterase antibody fixed on a quartz crystal reacts to acetylcholinesterase contained in a nerve sample to be measured, the oscillation frequency of the quartz crystal changes in the reaction process, then the content of the acetylcholinesterase is measured and mirrored through a computer monitor system, the nerve bundle to be measured with high content is of a motion tract, and the nerve bundle to be measured with low content is of a sensory tract. The invention starts with the difference of the content of the acetylcholinesterase in the peripheral nerve motion tract and the sensory tract, can fast detect an acetylcholinesterase piezoelectric biosensor system, fast discriminate the peripheral nerve motion tract and the sensory tract, and realize the correct sewing of the motion tract to the motion tract, and the sensory tract to the sensory tract. The invention can be widely popularized in the further clinical application.

Description

technical field [0001] The invention relates to a sensor for detecting the properties of peripheral nerve bundles, in particular to the application of an acetylcholinesterase piezoelectric biosensor in detecting the properties of peripheral nerve bundles. Background technique [0002] In the existing technology, there are mainly the following ways to judge the nature of the nerve bundle: [0003] 1. Anatomical method: To study the location map of the nerve bundles in the nerve trunk, many scholars have analyzed the peripheral nerves (such as the median nerve, radial nerve, ulnar and radial nerve trunk, sciatic nerve trunk, etc.) respectively, and observed the nerve trunk. The location, shape, and number of bundles of the nerve were measured, and the local location map of the nerve bundle was drawn. However, in clinical practice, the two ends of the nerve are often twisted after nerve injury, which changes the original normal anatomical position. Clinical applications are li...

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Problems solved by technology

However, this method needs to keep the patient awake, and is limited to fresh injuries, individual differences are large, and it is impossible to distinguish whether the muscle contraction caused by stimulation is the result of nerve conduction or current diffusion.

Therefore, the clinical application value is not large

[0005] 3. Radiobiochemical method: use radioactive isotope to label the substrate of the enzyme, and then measure the content of the enzyme according to the radioactive product produced by the enzyme reaction, and infer the nature of the nerve bundle, but this method involves isotope technology, high equipment requirements, and radiation The substance is harmful to both the patient and the operator, so it is not very practical

However, there are problems with this method: (1) the staining time is long (about 50 minutes), and the process is complicated; (2) two nerve segments at the stumps need to be cut out, and in situ staining cannot be performed

So far, there is no report on the piezoelectric biosensor for detecting the content of acetylcholinesterase

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