High-sensitivity mutation site detection system, high-sensitivity mutation site detection method and application
A detection method and system technology, applied in the field of biomedicine, can solve the problem of limited ability to detect rare gene mutations
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[0070] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.
[0071] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.
[0072] A highly sensitive gene mutation detection method (ASPB) of this embodiment includes: designing wild-type upstream or downstream primers for wild-type genes, mutant upstream or downstream primers for mutant genes, and universal upstream or downstream primers , mutant blocker and wild type blocker;
[0073] A double-tube PCR system is used for PCR reaction, and quantitative PCR detection is performed on samples; one tube includes wild-type primers, mutant blocker and universal primers, and the other tube includes mutant primers, wild-type blocker and universal primers.
[0074] Wherei...
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