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Method for evaluation of residual biopotency of antibiotics in waste water

A bio-efficacy, antibiotic technology used in the preparation of samples for testing, analysis by chemically reacting materials, and material analysis by observing the effect on chemical indicators

Active Publication Date: 2014-07-09
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to aim at the technical problems existing in the determination of the biological potency of existing antibiotic residues, and to provide a method for evaluating the biological efficacy of antibiotics in wastewater with universality. The residual bioefficacy of the wastewater of antibiotic metabolites of active groups can be accurately determined, the operation is simple, and the evaluation of the residual bioefficacy of different types of antibiotic wastewater is universal and universal

Method used

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  • Method for evaluation of residual biopotency of antibiotics in waste water
  • Method for evaluation of residual biopotency of antibiotics in waste water
  • Method for evaluation of residual biopotency of antibiotics in waste water

Examples

Experimental program
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Effect test

Embodiment 1

[0089] Example 1 Standard Antibiotic Screening

[0090] 1. Preparation of standard bacterial solution

[0091] Take out the frozen Gram-positive bacteria Staphyloccocus aureus (Staphyloccocus aureus) and Gram-negative bacteria Escherichia coli (Escherichia coli) from the -80°C refrigerator, thaw in a 4°C refrigerator, shake well, and add antibiotics respectively In No. Ⅲ culture medium. Put them into a shaking incubator at 37° C. and shake them until the absorbance of the culture solution at a wavelength of 530 nm is between 0.3 and 0.7 to obtain revived Staphylococcus aureus and revived Escherichia coli respectively.

[0092] In the present invention, the resurrection culture temperature of Gram-positive bacteria and Gram-negative bacteria is 35-37°C, which is suitable for the present invention; the culture time is 4-5h.

[0093] After sterilizing the inoculation loop, dip in the revived Staphylococcus aureus standard bacteria solution and the revived Escherichia coli stand...

Embodiment 2

[0144] Example 2 Determination of comprehensive biological efficacy of spiramycin production wastewater residues

[0145] The embodiment of the present invention takes spiramycin production wastewater as an example to describe in detail, and the treatment process of spiramycin production wastewater is as follows figure 1 As shown, the wastewater from spiramycin production passes through the regulating tank, the up-flow anaerobic sludge bed (UASB, Up-flow Anaerobic Sludge Bed / Blanket), the anoxic tank, the aerobic tank, and the secondary sedimentation tank in sequence, and reaches the level of "fermentation type". Discharge Standard for Water Pollutants in the Pharmaceutical Industry GB21903-2008", direct discharge, in which: SPM-1 is the influent water before entering the regulating tank; SPM-2 is the anaerobic effluent after anaerobic sludge bed treatment; SPM-3 is the The effluent treated by the anoxic tank; SPM-4 is the effluent treated by the aerobic tank; SPM-5 is the eff...

Embodiment 3-6

[0174] Example 3-6 Determination of comprehensive biological efficacy of spiramycin production wastewater residues

[0175] In addition to collecting wastewater water samples (SPM-2) from the effluent of the wastewater treatment workshop of the spiramycin production plant (SPM-2), anaerobic biological treatment from the wastewater treatment workshop of the spiramycin production plant Waste water samples (SPM-3) were collected from the effluent of the anoxic pool after the spiramycin production plant, wastewater samples (SPM-4) were collected from the effluent of the aerobic pool after aerobic biological treatment in the wastewater treatment workshop of the spiramycin production plant, and The wastewater sample (SPM-5) was collected from the outlet of the secondary sedimentation tank after the secondary biological treatment in the wastewater treatment workshop of the spiramycin production plant. The dilution factor of the wastewater sample (SPM-2) in Example 3 was 50 times. 200...

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Abstract

The invention discloses a method for evaluation of comprehensive biopotency (potency equivalent) of antibiotics in waste water, and the method comprises determination of absorbancy of the waste water by use of turbidimetry and calculation of the residual biopotency of the antibiotics in the waste water. According to the method, different antibiotics in the wastewater and antibiotic metabolites with antimicrobial activity groups can be s measured accurately, and the operation is simple, so that the method can be used for comparison of waste water with different types of antibiotics or comprehensive evaluation of mixed waste water with various antibiotics, and has universality on evaluation of the residual biopotency of the waste water with different types of antibiotics.

Description

technical field [0001] The invention belongs to the detection field of antibiotics, in particular to a method for evaluating the biological efficacy of residual antibiotics and related substances in antibiotic production wastewater. Background technique [0002] The pollution of antibiotics has received more and more attention. Antibiotics have become ubiquitous pollutants in the environment. They are not only frequently detected in soil and surface water, but also found in groundwater and even drinking water sources. Among them, antibiotic production wastewater is one of the important sources of antibiotics entering the environment. In 1995, Holm et al. analyzed the groundwater of an abandoned pharmaceutical factory waste landfill, and found that the concentration of sulfonamides reached 5 mg / L; in 2008, a survey in my country found that the concentration of oxytetracycline in the effluent after treatment of oxytetracycline production reached 50 mg / L. L. The residual antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/82G01N1/40G01N1/34
Inventor 张昱张红杨敏刘苗苗田哲高迎新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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