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Hemolytic agent, method for pretreatment of biological sample, method for determining content of target substance, and kit

A technology of biological samples and hemolytic agents, applied in the field of analysis, can solve problems that need to be improved, and achieve the effects of overcoming slow detection speed, improving detection efficiency, and promoting immune response

Active Publication Date: 2017-03-29
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current method of immunoturbidimetric assay still needs to be improved

Method used

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  • Hemolytic agent, method for pretreatment of biological sample, method for determining content of target substance, and kit
  • Hemolytic agent, method for pretreatment of biological sample, method for determining content of target substance, and kit
  • Hemolytic agent, method for pretreatment of biological sample, method for determining content of target substance, and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Prepare hemolyzing reagent A for whole blood C-reactive protein detection according to the following formula:

[0099] Triton X-114 8.0g

[0100] Boric acid 5.6g

[0101] Sodium 4-Hydroxyethylpiperazineethanesulfonate 2.5g

[0102] Add deionized water to dissolve, adjust an appropriate amount of NaOH or HCl to 7.0, and add deionized water to a final volume of 1L.

[0103] In this example, 8 microliters of a known concentration of C-reactive protein calibrator (concentration in the range of 0-320 mg / L) was added to 0.2 mL of hemolytic reagent A prepared. Under the condition that the temperature was kept at 37°C, the obtained mixture was allowed to stand for hemolysis for 90 seconds, and then 0.2 mL of C-reactive protein immunoassay reagent was added to the mixture, and after mixing, the change of the absorbance signal at 850 nm was measured to calculate the immunoassay. The change rate of turbidity (also referred to as "reactivity") ΔA=(A2-A1) / (T2-T1) from the 30th se...

Embodiment 2

[0115] Prepare hemolyzing reagent C for whole blood C-reactive protein detection according to the following formula:

[0116] Lauryl dimethyl betaine 0.05g

[0117] Boric acid 5.6g

[0118] Sodium 4-Hydroxyethylpiperazineethanesulfonate 2.5g

[0119] Dissolve the above components with deionized water, adjust to 7.0 with an appropriate amount of NaOH or HCl, and then add deionized water to a final volume of 1 L.

[0120] According to the method substantially the same as Example 1, the standard curve of hemolytic agent C is established, the difference is only that the hemolytic agent A in Example 1 is replaced by hemolytic agent C, and the standard curve of hemolytic agent C is shown in figure 2 middle.

Embodiment 3

[0130] Prepare hemolyzing reagent E for whole blood C-reactive protein detection according to the following formula:

[0131] Saponin 4.0g

[0132] Boric acid 5.6g

[0133] Sodium 4-Hydroxyethylpiperazineethanesulfonate 2.5g

[0134] Dissolve the above components with deionized water, adjust to 7.0 with an appropriate amount of NaOH or HCl, and then add deionized water to a final volume of 1 L.

[0135] According to the method basically the same as Example 1, establish the standard curve of hemolyzing agent E, the only difference is that the hemolyzing agent A in Example 1 is replaced by hemolyzing agent E, and the time of standing hemolysis after mixing the hemolyzing agent and the sample is shortened for 30 sec, the standard curve of Lysing Reagent E is shown in image 3 middle.

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Abstract

The invention discloses a hemolytic agent, a method for pretreatment of a biological sample, a method for determining the content of a target substance, and a kit. The invention provides a use of a boric acid buffer system in preparation of the hemolytic agent, the hemolytic agent for immunological turbidimetry determination, the method for pretreatment of the biological sample, the method for determining the content of the target substance in the biological sample, and the kit for immunological turbidimetry determination. The hemolytic agent is used for immunological turbidimetry determination. When the hemolytic agent prepared by the boric acid buffer system is used for immunological turbidimetry determination, the hemolytic agent has at least one of the following advantages of high sensitivity, fast and simple detection process and strong specificity.

Description

technical field [0001] The present invention relates to the field of analysis. Specifically, the present invention relates to a hemolytic agent, a method for pretreatment of a biological sample, a method for determining the content of a target substance, and a kit. More specifically, the present invention relates to the use of a boric acid buffer system in the preparation of a hemolytic agent, and for immunoturbidity The hemolytic agent for determination, the pretreatment method of biological samples, the determination method of target substance content in biological samples and the kit for immunoturbidimetric determination. Background technique [0002] The principle of immunoturbidimetric assay is: when the antigen and antibody react in a special dilution system and the ratio is appropriate (usually the antibody is excessive), the formed soluble immune complex will react with the polymerization accelerator (such as polyethylene glycol, etc.) Under the condition, it precip...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/82G01N33/53
Inventor 刘林张子千左淼陈庚文崔双
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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