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Immunodiagnosis kit for detecting IV-type dengue virus NS1 antigen

A technology for dengue virus and immunodiagnosis, applied in the field of medicine, can solve the problems of reducing dengue virus, inability to distinguish four serotypes of dengue virus, etc., achieving high sensitivity, conducive to early diagnosis and treatment, high sensitivity Effect

Active Publication Date: 2009-10-07
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Furthermore, these two kits cannot distinguish between the four serotypes of dengue virus. Since the clinical symptoms and treatment options caused by different serotypes of dengue virus infection are different, clinicians must determine which serotype is different as soon as possible. dengue virus infection for rapid and effective treatment
Therefore, timely determination of the type of dengue virus infection is a prerequisite for reducing the mortality rate of dengue virus infection and an effective measure to control the spread of dengue virus. However, none of the commercial kits currently on the market can achieve this goal.

Method used

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  • Immunodiagnosis kit for detecting IV-type dengue virus NS1 antigen
  • Immunodiagnosis kit for detecting IV-type dengue virus NS1 antigen
  • Immunodiagnosis kit for detecting IV-type dengue virus NS1 antigen

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Embodiment Construction

[0011] Below further illustrate kit of the present invention by concrete example and experimental report:

[0012] 1. The preparation method and identification results of the monoclonal antibodies 2E8A5 and 7A6A1:

[0013] (1) Preparation of immune antigen

[0014] The immunogen used to prepare the monoclonal antibody in the present invention is gene recombinant DV4NS1 protein and inactivated natural virus antigen. The gene recombinant DV4 NS1 protein is prepared by an engineering strain carrying the DV4 NS1 gene, and its preparation is carried out according to a conventional method, and the NS1 antigen is obtained by purifying with nickel primary nitrogen triacetate metal affinity chromatography, and the detailed preparation The method can refer to the user manual. After NS1 protein was purified, it was quantified with Coomassie protein assay reagent (PIERCE, Cat, No. ED62976). The results of Western blot identification of the purified recombinant protein showed that the m...

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Abstract

The invention discloses an immunodiagnosis kit for detecting specific IV-type dengue virus antigen, comprising a microporous reaction plate coated with monoclonal antibody 2E8A5, sample treatment fluid, monoclonal antibody 7A6A1 combined with markers, positive control, negative control, wash concentrate, color developing solution and stop solution. The kit can detect IV-type dengue virus antigen specifically, does not cross-react with other three types of serotype dengue virus antigens and detects that the supernatant sensitivity of the IV-type dengue virus is 64 times of that of the commercial kit of the same type, namely, the Pan-E Dengue Early ELISA test kit, thus greatly improving the sensitivity of detection of clinical serum samples and simultaneously reducing omission rate in clinical applications.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a medical preparation, in particular to a kit for diagnosing type IV dengue virus. Background technique [0002] Dengue fever is a tropical infectious disease transmitted by Aedes aegypti and Aedes albopictus. According to the antigenicity of E protein, it is divided into four serotypes, namely type I, type II, type III and type IV (referred to as DV1, DV2, DV3 and DV4). Any type of dengue virus infection can cause a series of clinical symptoms, manifested as occult infection, fever, dengue fever, or more severe dengue hemorrhagic fever and dengue shock syndrome. In recent years, due to the development of international tourism and improper prevention and control measures for infectious diseases, dengue fever is widely prevalent in more than 100 countries and regions in the tropics and subtropics, especially in Southeast Asia. At present, about 2.5 billion people in the world are threate...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577G01N33/535
CPCY02A50/30
Inventor 车小燕丁细霞
Owner SOUTHERN MEDICAL UNIVERSITY
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