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Protein chip for diagnosing early malignant tumor

A technology of protein chips and malignant tumors, applied in the field of protein chips, can solve the problems of inability to detect malignant tumors, PCR false positives, complicated operations, etc., and achieve the effects of saving national financial resources, strong specificity, and good repeatability

Inactive Publication Date: 2002-03-06
GENETECH BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, tumor markers are the one-by-one detection of the substances produced by tumor tissue or the body's response to tumor tissue, which has the disadvantages of complicated, repetitive, time-consuming, laborious, and expensive operations.
However, the recently released tumor-specific growth factor (Tumor Specific Growth Factor, TSGF) kit has a certain role in the early diagnosis of cancer, but its biggest disadvantage is that it cannot detect which part of the malignant tumor has occurred, and it needs the help of ultrasound. , MRI and other physical methods, or other organ-specific tumor markers in conjunction with detection to determine the location of the lesion
However, molecular biology detection requires repeated operations such as polymerase chain reaction (PCR), and PCR is prone to false positives, which is an unreliable method

Method used

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  • Protein chip for diagnosing early malignant tumor
  • Protein chip for diagnosing early malignant tumor
  • Protein chip for diagnosing early malignant tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Specific implementation method embodiment one, such as image 3 A liver cancer diagnostic protein chip is shown, where A1: anti-alpha-fetoprotein (AFP) antibody A2: anti-γ-glutamyl transpeptidase isoenzyme (γ-GT) I', II, II' antibody A3: Anti-abnormal prothrombin (DPC) antibody A4: anti-α-L fucosidase antibody B1: anti-5'-nucleotide phosphodiesterase isoenzyme V, VI antibody B2: anti-placental glutathione S Transferase antibody B3: anti-α1-antichymotrypsin antibody B4: anti-ferritin antibody C1: anti-acid isoferritin antibody C2: anti-hepatitis B surface antigen antibody C3: hepatitis C antigen D1: positive control D2: negative control

[0019] So far, AFP is still the main experimental indicator for the diagnosis of liver cancer. However, in some benign liver diseases, teratomas of the reproductive system, and some malignant tumors of the gastrointestinal tract, AFP can also be elevated, while in other patients with hepatocellular carcinoma, AFP is always low...

Embodiment 2

[0021] Example two, such as Figure 4 As shown in the pancreatic cancer diagnostic chip,

[0022] A1: Anti-CA19-9 antibody

[0023] A2: Anti-CA242 antibody

[0024] A3: Anti-CA50 antibody

[0025] B1: Anti-CA72-4 antibody

[0026] B2: Anti-CA125 antibody

[0027] B3: Anti-tissue synthetic peptide antigen antibody

[0028] C1: Anti-carcinoembryonic antigen (CEA) antibody

[0029] C2: Anti-Peptide Carcinoma Peptide Antigen (POA) antibody

[0030] C3: Anti-galactosyltransferase isoenzyme II (GalTII) antibody

[0031] D1: Positive control

[0032] D2: Negative control

[0033] The same as the above-mentioned production method, the clinical detection type malignant tumor diagnostic protein chip for other organs can be designed.

Embodiment 3

[0034] Embodiment 3 The protein chip for the early diagnosis of general survey-type malignant tumors is formed by combining two or more known indicators of the clinical detection-type malignant tumor diagnosis protein chip.

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Abstract

A protein chip for diagnosing the early-phase malignant tumor is prepared by arraying the label antigens of different tumors, positive references (the marker of relative tumor) and negative references (human serum albumin) in a same cavity of protein chip by full-automatic high-speed robot, and the protein is linked to the solid carrier by chemical bonds. Its advantages are high sensitivity and specificity, high stability and repeatability of result, high correctness and efficiency.

Description

Technical field [0001] The protein chip for early diagnosis of malignant tumors of the present invention relates to a protein chip. The protein chip technology is an emerging technology that arranges proteins or polypeptides on a solid substrate in an orderly manner, and uses immunological methods to detect, identify and identify proteins. It has the characteristics of small size, high information content, and combination design according to different needs. Background technique [0002] In recent years, malignant tumors have become common and frequently-occurring diseases that endanger human health. It not only threatens the lives of patients, but also has high treatment costs. However, malignant tumors are not incurable. As long as they are detected and treated as early as possible, the cure rate can reach more than 90%, and the cost of early treatment is relatively low. Therefore, many scientists in the world are committed to early diagnosis of malignant tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/96
Inventor 张涛李宾彭永济李红梅任一萍
Owner GENETECH BIOTECH SHANGHAI
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