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Single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and application thereof

A technology for susceptibility and liver cancer, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of no relevant detection methods and detection kits, no STAT4 gene, etc.

Inactive Publication Date: 2014-06-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report on the association between the STAT4 gene involved in the present invention and liver cancer, nor the correlation between polymorphic sites on the STAT4 gene and liver cancer, as well as the detection method and detection kit for specific SNPs.

Method used

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  • Single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and application thereof
  • Single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and application thereof
  • Single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Genotyping of the SNP site rs9275319 was performed by extracting the genomic DNA of the subjects, and the individuals whose rs9275319 base was A were the susceptible population of liver cancer. The specific method is:

[0038] 1. Genomic DNA Extraction

[0039] All subjects were drawn 2-3ml of peripheral venous blood, and genomic DNA was extracted using QIAamp Blood MiniKit250 (QIAGEN, Germany) kit, and stored at -20°C.

[0040] 2. SNP genotyping

[0041] According to the sequence information of SNP, PCR reaction and single-base extension primer (ie UEP primer) were designed and synthesized in Bioengineering (Shanghai) Co., Ltd. The synthesized primers were subjected to PCR reaction with the sample DNA, and the mixed reaction product was subjected to SNP genotyping on a SequenomiPLEX instrument (Sequenom Company). The specific experimental process is as follows:

[0042] (1) Primer design: automatically design PCR primers for multiplex reactions (SEQ.ID.NO.3; SEQ.ID...

Embodiment 2

[0069] Through a multi-stage case-control study, this study shows that the SNP site rs9275319 on the 6p21.3 region is associated with liver cancer. The study took 5480 cases of hepatitis B patients diagnosed with liver cancer as the research group, and 6139 cases of hepatitis B patients without liver cancer as the control group. Genomic DNA was extracted from all subjects and stored at -20°C.

[0070] The results of the multi-stage case-control study showed that those with rs9275319 base A had a high risk of developing liver cancer, as shown in the table below:

[0071]

[0072]

[0073] It can be seen that the genotyping method for the SNP site rs9275319 of the present invention has high accuracy, good repeatability and reliable results.

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Abstract

The invention belongs to the gene field of biotechnology, and relates to a single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and an application thereof. The invention provides a gene partial sequence (the sequence is shown by SEQ ID No.1) of the genetic area 6p21.3 and an SNP site rs9275319 (the sequence is shown by SEQ ID No.2). The invention also provides a method for detecting the SNP site, a specific nucleic acid primer with sequences shown by SEQ ID No.3 and SEQ ID No.4, and a UEP primer with a sequence shown by SEQ ID No.5. The invention also relates to a detection kit for the SNP site rs9275319 and an application thereof. In the invention, genotyping can be performed on the rs9275319 site, the method is simple and quick, the result is accurate and clear, and a new means is provided for the liver cancer susceptibility identification as well as prevention and treatment.

Description

technical field [0001] The invention belongs to the gene field of biotechnology, and specifically relates to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site related to liver cancer susceptibility and its application. Background technique [0002] Liver cancer is the seventh most common malignant tumor in the world, ranking third among cancer-related causes of death (Yang JD, Roberts LR. Hepatocel lular carcinoma: A global view. Nat Rev Gastroenterol Hepatol. 2010; 7:448-58.), It is estimated that there are 748,300 new cases and 695,900 deaths in the world every year, and more than half of the liver cancer cases are concentrated in China (Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics . CA Cancer J Clin. 2011;61:69-90.). [0003] Worldwide, chronic hepatitis B and chronic hepatitis C are the main risk factors for liver cancer. Almost 80% of liver cancer patients have a history of hepatitis B or C, and 54% of liver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 余龙蒋德科徐剑锋马晓颦唐丽莎
Owner FUDAN UNIV
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