Rubber tree HbAG gene and application thereof
A rubber tree and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of long sexual generation cycle, limitation of rubber tree breeding efficiency, unsuccessful combination of hybrid combinations, etc. The effect of improving breeding efficiency
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[0019] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. The experimental method that does not indicate specific experimental condition in the following examples, generally according to conventional conditions, molecular cloning (Molecular Cloning: A Laboratory Manual, 3rd ed.) or yeast genetic method experiment guideline (Methodsin Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual , Adams A et al edited, Cold Spring Harbor Laboratory, 1998 publication), or according to the conditions suggested by the manufacturer.
[0020] 1. Cloning of Hevea HbAG gene
[0021] (1) Take the root, stem, stem tip, leaf, xylem, latex, fruit, inflorescence, male flower and female flower of the rubber tree and refer to Invitrogen The steps of the method for extracting RNA by Reagent are carried out to obtain total RNA that meets the requirements of subsequ...
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