Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala

Abstract

A method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala includes the following steps that firstly, female cyprinus carpio and male megalobrama amblycephala which have obvious sexual maturity characteristics and good somatic features are selected as parent fishes for special pool breeding, then, artificial spawning induction is conducted on the parent finishes in a breeding season, the parent fishes with a good spawning induction effect are selected to be subjected to artificial dry-method fertilization, running water incubation is conducted on fertilized embryos in a pure water incubation device, fish fry are raised after being incubated, the raised fish fry are detected and screened, hybrid offspring of the cyprinus carpio and the megalobrama amblycephala are obtained through flow cytometry DNA content determination and renal tissue chromosome ploidy detection, and the hybrid offspring comprise diploid natural gynogenesis cyprinus carpio, diploid natural gynogenesis mirror carps, diploid fish like the cyprinus carpio and tetraploid hybrid fish. The method has the advantages that the rate of fertilization is high, the incubating rate is high, the offspring are excellent in character, and the method has great significance in genetic breeding.

Description

technical field [0001] The invention relates to a fish hybridization method, in particular to a distant hybridization method among subfamilies of carps. Background technique [0002] Content Determination The hybridization of genetic relationship between species or above is called distant hybridization, which is a commonly used method for fish trait improvement. As an important method of fish genetic breeding and trait improvement, distant hybridization can integrate a whole set of exogenous genes, thereby changing the expression regulation of hybrid progeny genes, so that hybrid progeny may improve in growth speed, stress resistance, disease resistance and Meat quality and other aspects showed heterosis. Distant hybridization can form different types of fish with different phenotypes and genotypes according to different combinations of parents. For example, F 1 -F 2 For diploid hybrid fish (2n=100), F 3 -F 24 It is an allotetraploid fish (4n=200); using allotetraploid...

AI Technical Summary

Problems solved by technology

So far, there have been no reports of successful distant hybridization between carp and roundhead bream at home and abroad. It is of great application value to explore a hybridization method between carp and roundhead bream and to study and analyze the biological characteristics of their offspring.

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