Rice dwarf straw leaf roll mutant (cd1) gene and application thereof
A mutant and gene technology, which is applied to the leaf-rolling dwarf stalk mutant gene and its application field, can solve the problems of single dwarf stalk gene in rice breeding, lack of experimental evidence for the clear function of the gene, affecting the yield of rice bred varieties, and the like. The content is reduced, and the effect of drought resistance is improved
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] 1. Materials and methods
[0024] 1. Materials
[0025] Mutants were derived from the A c / Ds insertion mutant library constructed in our laboratory (Luan et al., 2008) 15 , for the background of Oryza sativa L.ssp.Japonica cv.Nipponbare. The mutants were crossed with Oryza sativa L. ssp. Indica cv. Nipponbare to produce the F1 generation, and the F1 generation was self-crossed to generate the F2 generation segregation population.
[0026] All materials were planted in the experimental field of China Rice Research Institute.
[0027] 2. Method
[0028] 2.1 Micro-extraction of rice genomic DNA
[0029] Extracted by simple CTAB method. Specific steps are as follows:
[0030] 1) Take about 300mg of fresh young rice leaves, freeze them with liquid nitrogen, grind them into powder, and quickly transfer them to a 1.5mL Enpdoff tube;
[0031] 2) Add 650 μL of CTAB extraction buffer (100mM Tris-HCl pH 8.0, 20mM EDTA, 1.4M NaCl, 2.0% CTAB, 1% PVP) preheated at 65°C, bathe ...
Embodiment 2
[0138] 3. Prediction of the application prospect of the cloned target gene
[0139] Polysaccharides play an important role in the development of plant cells, mainly including cellulose, hemicellulose and pectin. On the one hand, these polysaccharides are part of cell morphogenesis; on the other hand, they play important roles in the environment and physiology of cell survival. The synthesis and metabolic pathways of these polysaccharides are very complex. It is currently known that glycosyl transferases (GTs) play an important role in the synthesis of polysaccharides. The gene encoding this enzyme is a superfamily of which CSLDs are among them. A subfamily of CSLDs, in existing reports, the function of CSLDs subfamily is not very clear, in Arabidopsis, it is related to the synthesis of xylan and homopolygalacturonic acid, which affects the development of stems. OsCSLD1 was reported to be involved in the development of root hairs in rice. In this study, a dwarf leaf-rolling m...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com