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Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino

A technology of head cabbage and tetraploid, applied in the directions of botanical equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., to achieve the effects of high probability, high seed setting rate, and extensive genetic diversity

Inactive Publication Date: 2011-01-19
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, there is no use of colchicine to induce diploid non-heading Chinese cabbage to produce 2n male gametes in the prior art at home and abroad, and then cross with tetraploid non-heading Chinese cabbage cytoplasmic male sterile lines to obtain new tetraploids. sexual polyploidy breeding technology

Method used

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  • Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino
  • Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino
  • Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino

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Embodiment 1

[0034] 1 Materials and methods

[0035] 1.1 Materials

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Abstract

The invention belongs to the field of crop breeding and discloses a sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino. The method comprises the following steps of: treating flower buds by using solution of colchicine in the bolting period of the Brassica campestris ssp.chinensis Makino to induce the flower buds to generate 2n male gametes; and performing manual sexual polyploidization cross breeding on a tetraploid male sterile line serving as a female parent and 2n male gamete-containing diploid obtained by the induction of the solution of colchicine serving as a male parent in a blooming period to obtain the tetraploid Brassica campestris ssp.chinensis Makino. In the method, a sexual polyploidization breeding approach of hybridizing the male sterile line of the tetraploid Brassica campestris ssp.chinensis Makino with the diploid Brassica campestris ssp.chinensis Makino which generates 2n gametes after induction is used for the first time to obtain a new species of the tetraploid Brassica campestris ssp.chinensis Makino which has wider genetic diversity and higher seed setting rate compared with the autotetraploid of asexual polyploidization. The new tetraploid of the invention has the characteristics of polyploid such as largeness, disease resistance and cold resistance, high quality, and the properties of a diploid male parent.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a sexual polyploidy breeding method for tetraploid non-heading cabbage, which is specially used for the breeding of tetraploid non-heading cabbage. technical background [0002] There are two polyploidization pathways in the evolution of species in nature. One is sexual polyploidy, and the other is asexual polyploidy. The former is the main way to form polyploidy in natural plants, that is, through the formation of 2n gametes, and then participate in sexual mating. Sexual polyploidy of 2n gametes has more biological advantages than asexual polyploidy, with higher frequency and higher heterozygosity. From introgression from closely related diploids, genetic diversity is created and polyploid populations that are genetically heterozygous are created. Polyploid breeding with 2n gametes enables different genotypes to combine at increased ploidy levels, providing the possibility to transm...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/08A01H5/00
Inventor 张蜀宁李英侯喜林钟程于旭红李式军刘惠吉
Owner NANJING AGRICULTURAL UNIVERSITY
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