Excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique

Abstract

The invention provides an excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique, wherein dinitro toluene herbicide (DNH) is employed to substitute the conventional colchicines as inducer, whose function is to suppress the Mitosis in the metaphase of cell division through the mechanism of interfering spindle, so as to double the tissue cell chromosome. The method has the advantages of increased inducement success rate and substantially shortened time required for inducement.

Description

technical field [0001] The invention relates to a watermelon in vitro mutagenesis tetraploid method and early ploidy identification technology. Background technique [0002] Triploid seedless watermelons are obtained by crossing tetraploid and diploid watermelons. In order to obtain excellent triploid seedless watermelon varieties, the key point is to obtain tetraploid materials through chromosome doubling of diploid watermelons. Since Kikara reported the research on the breeding of triploid seedless watermelon in 1951, many researchers have successively carried out the research of using colchicine (Colchicine) to induce tetraploid watermelon. There are mainly the following ways to obtain tetraploid watermelon: (1) Excavate natural variation from nature. Some natural phenomena such as lightning, air, drastic temperature changes, chemical substances in the soil, and trauma to watermelon plants can sometimes induce tetraploidy. (2) Induction by physical methods: use various...

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Problems solved by technology

Herbaceous plants such as watermelon have poor regenerative ability during in vitro culture, and the use of colchicine in the initial regeneration culture will significantly inhibit the regeneration of adventitious buds, which will bring many difficulties to in vitro doubling

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