56 results about "Spindle apparatus" patented technology

A system, apparatus and method is shown for collecting data on rolled product usage at a dispensing location by monitoring the amount of product pulled or removed from a roll. In the invention, a spindle assembly may be configured to support a product roll. The spindle assembly includes a rotating member in operable connection to the spindle. A sensor is connected to the spindle assembly. The sensor is capable of detecting one or more parameters of product usage from the roll. Furthermore, the sensor may be configured to measure at least the degree of movement of the rotating member during removal of product from the roll. A recording device also may be provided for receiving data from the sensor, the recording device being adapted for receiving and recording data. Data that may be generated, and may be recorded, includes roll product usage data. An electronically controlled system of monitoring product inventory, and / or ordering more product when inventory falls below a predetermined threshold amount is disclosed.

The invention provides an excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique, wherein dinitro toluene herbicide (DNH) is employed to substitute the conventional colchicines as inducer, whose function is to suppress the Mitosis in the metaphase of cell division through the mechanism of interfering spindle, so as to double the tissue cell chromosome. The method has the advantages of increased inducement success rate and substantially shortened time required for inducement.

Even when there is some degree of variation in the characteristics among components constituting a polyphase motor and a driving circuit therefor, current control signals of respective phases being input to the drive circuit have their amplitude finely adjusted by an amplitude control circuit, so that amplitude is uniform among armature currents of respective phases that are ultimately output from the drive circuit. The amplitude adjusting circuit is configured by gain variable amplifiers, for example. Thus, rotation fluctuation and vibration of the polyphase motor can be reduced.

A system, apparatus and method collects data on rolled product usage at a dispensing location by monitoring the amount of product pulled or removed from a roll. A spindle assembly may be configured to support a product roll. The spindle assembly includes a rotating member disposed about a sensor. The sensor is capable of detecting one or more parameters of product usage from the roll. Furthermore, the sensor may be configured to measure at least the degree of movement of the rotating member during removal of product from the roll. A recording device also may be provided for receiving data from the sensor, the recording device being adapted for receiving and recording data. Data that may be generated, and may be recorded, includes roll product usage data. An electronically controlled system of monitoring product inventory, and / or ordering more product when inventory falls below a predetermined threshold amount is also included.

The invention discloses an application of melatonin in preparation of a medicine for improving oocyte quality. According to the invention, melatonin is directly added into an oocyte in-vitro maturation culture solution, which proves that the melatonin can relieve the reduction of the quality of the cotinine-induced oocytes and is shown as reduction of the ROS level in the oocytes, reduction of aneuploidy formation, promotion of uniform distribution of actin, improvement of the form of spindles and improvement of mitochondrial functions, and then the later fertilization rate of the oocytes is remarkably increased. The invention provides a method for improving the influence of the main metabolite cotinine of nicotine in the blood of a smoker on the quality of female oocytes, and provides aneffective method for improving the reproductive capacity of smoking women.

The controlling device of the yarn twister is provided with a spindle apparatus 1, a spindle-driving motor 10, a spindle changer, a winding motor, a slowdown and stop controlling means 74 for the spindle driving motor, and a slowdown and stop controlling means 73 for a drum driving motor. The delay angle of the spindle apparatus 1 is included in the parameters for setting the slowdown and stop rate of the spindle driving motor 10 or the drum driving motor 20 controlled by the slowdown and stop controlling means 74 for the spindle driving motor or the slowdown and stop controlling means 73 for the drum driving motor.

Disclosed is a method for cloning buffalo's body cells comprising the following steps: charging calf follicular fluid and epithelium cell growth factor into maturation to cultivate acceptor oocyte, enucleating under spindle image system, cultivating and processing donor cells with DNA synthesized depressant Aphidicolin, carrying out hungry cultivation, activating the recombinant embryo with ionomycin and 6-dimethoxy aminopurine, and proceeding embryo extracorporal cultivation with modified TCM 199 and estrus cow's serum.

The invention relates to a two-for-one twister capable of detecting spindle speed and judging yarn state and a working method thereof; the two-for-one twister includes a nose controller; the nose controller is connected with a switch power supply, a touch screen and an alarm light; the nose controller is connected with a row of middle acquirers in differential transmission mode of an RS485 communication port, each middle acquirer is communicated with the switch power supply through a 24 V direct-current power supply, the switch power supply is connected with a 220 V alternating-current power supply, the switch power supply converts 220 V alternating current power into 24 V direct current power for the twister, the nose controller may read data in each middle acquirer through the RS485 communication port so as to acquire yarn state and spindle speed information of the whole twister, and to display information on the touch screen and control the lights. The function of automatically detecting spindle speed and judging yarn state can be achieved herein, and manual inspection on machining is not required during working.

The invention discloses a method for evaluating the influences of smoking on the quality of oocytes. Specifically, oocytes are cultured to be mature in culture solutions containing different concentrations of cotinine. Finally, the influences of smoking on the quality of oocytes are evaluated by detecting some indicators, such as the maturation rate of oocytes, chromosomal aneuploidy, actin distribution, mitochondrial membrane potential, intracellular reactive oxygen species level, spindle morphology, chromosome arrangement of mature oocytes, sperm binding number after in-vitro fertilization,pronucleus formation rate after parthenogenetic activation and the like. Through a large number of objective tests, the influences of smoking on the quality of oocytes are evaluated, thus providing areference of theoretical data for the maintenance of reproductive capacity of female smokers.

A photoelectrochemical immunosensor for detecting a dairy product allergen beta-lactoglobulin (beta-LG) is constructed, an Ag2S sensitized spindle-shaped BiVO4 / BiOBr heterojunction is fixed on the surface of an ITO (indium tin oxide) electrode by using an antibody for specifically recognizing allergen beta-LG to be used as an excellent photoactive substrate, so that the photocurrent response and the sensitivity are effectively improved. Thioglycollic acid TGA is used as a connecting agent to fix the beta-LG antibody, photocurrent signals of antigens with different concentrations are detected through specific binding between the antigens and the antibody, and the quantitative test of the beta-LG is completed. Under the optimal experiment conditions, the PEC immunosensor is good in stability, wide in linear range, high in sensitivity and good in reproducibility, and can be used for detecting actual samples. The label-free immunosensor has very strong capability of specially identifying beta-LG, is not influenced by other proteins in milk, and does not need pretreatment. Meanwhile, the developed immunosensor provides a promising PEC detection platform and a reference thought for detection of other proteins in milk.

The present invention relates to a spindle apparatus 100 for use on a numerically controlled machine tool, which spindle apparatus 100 has the following: a spindle housing 1, and a spindle shaft 15 which is mounted in the spindle housing, the spindle apparatus 100 having, furthermore, an electrically earthed section 4 which holds at least one rod element 20 which comprises an electrically conductive material, and the at least one rod element 20 being arranged in such a way that it makes contact with the spindle shaft 15 so as to bear tangentially against an outer circumference of the spindle shaft 15.

A two-for-one twisting or cabling spindle apparatus (1) with a supply bobbin (4) mounted on a pot base (2) of a stationary protective pot (3) and a spindle (6) arranged below the protective pot (3) to guide a yarn (5) to be processed during a twisting or cabling process. The spindle (6) is rotatably mounted by a lower bearing device (7) in a bearing housing (9) fixed on a spindle rail (8) and an upper bearing device (11) mounted to the stationary protective pot (3). The largest diameter (d) of the rotatably mounted spindle (6) is at least 10% less than the diameter (D) of a touching line (23) of the pot base (2) of the stationary protective pot (3) with the yarn (5).

The invention discloses synthesis and photocatalytic application of a spindle-shaped MIL-101 (Fe) photocatalyst, and belongs to the technical field of nano materials. According to the invention, ferric chloride hexahydrate is used as an iron source, terephthalic acid is used as a ligand, and MIL-101 (Fe) with special morphology is synthesized by using a solvothermal method in ethylene glycol and N,N-dimethylformamide (DMF) solvents. The preparation method is simple and easy to implement, economical and environmentally friendly, and the photocatalytic nitrogen fixation ammonia yield of the prepared MIL-101 (Fe) photocatalyst with the special morphology is 2.6 times that of traditional MIL-101 (Fe). Fe in the MIL-101 (Fe) photocatalyst with the special morphology plays a role in activating nitrogen and improving the photo-reduction capacity of the photocatalyst, the content of iron on the surface is increased through the special morphology, and the morphology is beneficial to reflection and refraction of light, so that the photocatalytic activity is improved, and the MIL-101 (Fe) photocatalyst has important significance in improving the nitrogen reduction efficiency.

The invention discloses an aloe active substance. 40 to 50 parts of waste syrup and 200 to 300 parts of aloe powder without essential oil are anaerobically fermented through utilizing an EM bacteriumagent. The aloe active substance prepared by the invention has the beneficial effects that sterilization is carried out through two ways: 1) smooth proceeding of cellular respiration is inhibited andgeneration of active oxygen free radicals is initiated; phospholipid and other large biological molecules are oxidized so that mitochondria and plasma membranes are damaged; then nucleotide flows outand the death of microorganisms is caused; 2) the metabolism of microorganism protein is interfered and spindle microtubule protein polymerization is inhibited; meanwhile, the activity of RNA (Ribonucleic Acid) polymerase also can be inhibited; the synthesis of cell membrane lipids and the transportation of amino acids on cell membranes are interrupted, so that mitosis of the microorganisms is stopped at a metaphase and mass propagation of the microorganisms is inhibited.

The invention relates to a reagent system and a reagent kit for detecting target genes of colorectal cancer spindle apparatus assembly checking point and application thereof. The reagent system comprises a reagent used for detecting BubR1 gene, a reagent used for detecting Mad 1 gene, a reagent for detecting Mad 2 gene, a reagent for detecting AURKB gene, a reagent for detecting PIK 1 gene and a reagent for detecting Cdc20 gene. According to the reagent system and the reagent kit for detecting target genes of colorectal cancer spindle apparatus assembly checking point and the application thereof, specially designed probe and PCR amplification technology are adopted, and through DNA specific binding reaction, the probe and the PCR amplification technology conduct one-time detection on expression state of six genes related to spindle apparatus assembly checking point during a last phase of a mitosis process, thus filling the blank of colorectal cancer detection in genes related to the spindle apparatus assembly checking point. The reagent system and the reagent kit are comprehensive and reliable in detection result, quick and efficient in effect, simple to operate, high in sensitivity, strong in specificity, low in misdiagnosis rate, and is capable of conducting timely precise diagnosis on generation and development of the colorectal cancer in earlier days.

The invention belongs to the technical field of biological medicine, and relates to a method for improving in-vitro and in-vivo maturation quality of oocytes and application of the method. Melatonin is directly added into an in-vitro maturation culture solution of the oocytes, female animals take in the melatonin, and the melatonin is proved to be capable of relieving quality reduction of the oocytes induced by oxybenzophenone. The application has the advantages that the in-vitro and in-vivo maturation rate of oocytes and the development rate of early embryos are increased, the spindle body morphology and the mitochondrial function are improved, the ROS level in the oocytes is reduced, the GSH level is increased, and the early apoptosis level is inhibited. The invention provides a method for improving the influence of oxybenzophenone on the quality of female animal oocytes, and the method has important significance for diagnosis and treatment of patients with abnormal maturation of oocytes caused by environmental factors clinically and in-vitro production of animal embryos.

Provided is a spindle apparatus having a good waterproof function. At least a portion of a rotating member 40, which is fixed to a rotary shaft 11 of the spindle apparatus 10 in an integrally rotatable manner and forming a portion of a waterproof mechanism for preventing a liquid from entering the front bearings 50, 50, is formed of a carbon fiber composite material.

The invention discloses a CENP-E binding proteins, coding gene thereof and application therefor. The objective is to provide a CENP-E binding proteins, coding gene thereof and application in preparing medicine for repressing tumour proliferation. The protein is one amino acid sequence of followings: 1) SEQ ID NO: 1 in sequence form; 2) protein for substituting, absenting or adding one to ten amino acid residue to SEQ ID NO: 1 in sequence form and combining with CENP-E proteins to regualte mitotic cell. The protein reacts with CENP-E and micropipe to participate control point transduction pathway of cell mitotic spindle, using thereof as active component as drug targets for regulating tumour cell proliferation, the proteins and coding gene of the invention have improtant function in medicine and pharmacy field with widely application foreground.

The present invention provides a buffalo oocyte enucleation method which comprises the following steps: 1) a cumulus oophorus oocyte cell complex is taken form ovary of a slaughter house for in vitro maturation culture for 22-24h under the conditions of 38.5 DEG C, 5% CO2 and saturated humidity; 2) the maturation-cultured COCs are gently and slowly blew and beaten by 100muL pipette tip for removal of cumulus oophorus cells to left oocytes; and 3) selecting MII oocytes containing first polarbody for enucleation; 4) transferring the buffalo oocytes into a culture solution containing 7.5 mug / mL cytochalasin B (CB) for culture for 30min under the conditions of 38.5 DEG C, 5% CO2 and saturated humidity; 5) transferring the treated buffalo oocytes into 30-40 muL of an enucleation operating liquid covered with paraffin oil; and 6) enucleating in an inverted microscope provided with a spindle body imaging system. By use of the cytochalasin B (CB) for culture of the oocytes and use of the spindle body imaging system for auxiliary enucleation, the efficiency of enucleation can be effectively improved.

The invention provides a preparation method of a lung cancer stem cell vaccine for obviously improving immunocompetence. The method comprises the following steps of (1) culturing the recovered lung cancer stem cells in a DMEM whole culture medium, wherein the DMEM whole culture medium comprises 8 percent of FBS; the culture temperature is 37 DEG C; the humidity is 85 percent; the CO2 concentration is 0.5 percent; the culture time is 3 to 4 days; then, performing 1:3 dish separation on the cultured cell; next, performing culture for 3 days by using the same culture medium under the same culture conditions so that the cells show spindles or are similar to spindles; (2) performing irradiation treatment by 60Co at the dosage of 3 to 4 Gy; (3) selecting CD133 positive stem cells; performing inactivation treatment after the culture; resuspending the stem cells by normal saline so that the cell concentration is 1*10<5> to 1*10<6> / ml; (4) according to the volume ratio of 4:1.5:2, mixing the materials obtained in the step (3), chitosan normal saline solution and laminarin normal saline solution, and after the mixing, the chitosan concentration is 2 percent, and the laminarin concentration is 2.5 percent. The obtained vaccine has the obvious curative effect on the lung cancer.

The invention pertains to the discovery that the presence of oocyte spindle associated factors in an enucleated oocyte improves oocyte quality and subsequently nuclear transfer. In particular, it was discovered that maintaining oocyte spindle factors in the oocyte after enucleation improves oocyte quality for use in nuclear transfer methodology.

The invention discloses a device for polishing parts with lace for construction mechanical equipment, which comprises a device body, a base is arranged on the device body, a support column is arranged above the base, and the support columns are left column, middle column and right column from left to right. The top of the support column is provided with a beam, the left part of the device body is provided with a slider A, the inside of the slider A is provided with a fixed rod, the right part of the device body is provided with a slider B, the device body is provided with a screw, the device body is provided with a support plate, and the support plate The motor is arranged at the bottom of the bottom, and the grinding wheel is also arranged at the bottom of the bottom; the invention has a simple structure, can effectively polish the parts, and is very convenient to adjust during grinding, and the device body can also be fine-tuned during the grinding process, so that the device body can be polished It can grind different positions of the parts with lace at the same time, the grinding effect is good, the grinding speed is fast, the efficiency of manual grinding is doubled, the use is convenient, and the practicability is strong.

The invention relates to an automatic ICSI system capable of accurately identifying, which comprises a power supply module, a main control module, a sample optical observation module, a user interaction module, a sperm capturing unit, an ovum fixing unit and a single sperm injection unit, the sample optical observation module, the user interaction module, the sperm capturing unit, the ovum fixing unit and the single sperm injection unit are all electrically connected with the main control module, and the operation method of the system comprises three steps of sperm capturing, ovum fixing and single sperm injection. According to the automatic ICSI system capable of accurately identifying and the operation method, the system can replace manual work to realize automation, is stable and reliable in experimental structure, adopts an AI image system to identify healthy and high-quality sperms and intelligently identify the spindle body part of an ovum, can perform needle insertion and injection on the best-quality sperms through the most appropriate position, greatly reduces the damage to the ovum, and the probability of various fertilization failures or embryo development potential underground caused by human factors is reduced to the greatest extent.

The invention provides a tension regulating device for embedded spinning, which can ensure that the tension of two filaments is consistent, the equipment occupies a small area, is convenient to set up, and unwinds the filaments smoothly. It includes a creel, four sets of spindle hanging devices are arranged on the horizontal bars of the creel, and upper and lower yarn guide devices are arranged on the vertical bars of the creel; filaments are arranged below the spindle hanging devices. Tension adjustment device; the filament tension adjustment device includes front and rear positioning rods arranged at equal heights, an adjustment rod is arranged above the front and rear positioning rods, and the front and rear positioning rods are parallel to the adjustment rods Setting; a front drafting device is arranged below the filament tension adjusting device.

Disclosed are a small molecule inhibitor Syntelin against kinetochore motor protein CENP-E and use thereof. Provided is a small molecule organic compound that binds to the motor domain of CENP-E motor protein. The binding of the compound to CENP-E inhibits the movement of CENP-E motor protein along microtubules without influencing its interaction with microtubules. The compound can enter the cells to inhibit the function of CENP-E when added to a cell culture medium, leading to partial error in chromosome arrangement and maintaining the spindle checkpoint activity for a long period of time.