Wild-rice distant hybridization high-efficient cultivating superior progeny method

Abstract

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

Description

technical field [0001] The invention relates to plant cultivation technology, in particular to a method for efficiently cultivating excellent offspring by distant hybridization of wild rice. Background technique [0002] Rice distant hybridization is the hybridization between rice families with different genome types in terms of botanical morphological classification, called rice distant hybridization. Distant hybridization breaks down the boundaries between species to a certain extent. Rice belongs to the genus Oryzae of the family Poaceae, and 23 species have been found. There are 3 kinds of wild rice distributed in China, and there are 3 kinds of wild rice in China in Yunnan, namely common wild rice, medicinal wild rice and verrucous wild rice. [0003] Distant hybridization of rice is the use of wild rice and cultivated rice to exchange genes artificially, so as to integrate different excellent and unique traits into a hybrid individual to varying degrees to create new...

AI Technical Summary

Problems solved by technology

The karyotype of common wild rice is A'A'; the karyotype of wild rice is CC; the karyotype of wild rice is GG, while the karyotype of cultivated rice is AA. It is very difficult to hybridize rice and wild rice, it is difficult to receive hybrid seeds, and it is also difficult to stabilize the traits after hybridization

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