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Method for inducing gynogenetic diploid of grouper

A technology of gynogenesis and grouper, which is applied in the field of induction of grouper gynogenetic diploids, can solve the problems of short breeding cycle, long breeding cycle, slow homozygosity of breeding model genes, etc., and achieve accelerated pure The effects of chemical synthesis and broad promotion and application prospects

Inactive Publication Date: 2010-12-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of excellent hereditary character can be fixed rapidly, the grouper gynogenetic two of grouper with short breeding cycle according to the problems such as slow homozygous speed of grouper traditional breeding model gene in the prior art, long breeding cycle etc. Induction method of ploidy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] One day in advance, the male and female grouper were injected with oxytocic agents, such as chorionic gonadotropin HCG and luteinizing hormone-releasing hormone analogue A2. Anesthetize the female fish and squeeze eggs according to the situation. Before the eggs were obtained, the grouper males were anesthetized and the semen was squeezed. Dilute the semen 5 times with the diluent, place it in a petri dish with a diameter of 9-15cm, and irradiate it with ultraviolet light at a dose of 800×100μW / cm 2 ; Genetic inactivation of spermatozoa. Mix processed sperm with eggs to a final sperm concentration of at least 10 5 per ml, add seawater at a temperature of 25°C to start the gynogenesis of grouper eggs. Three minutes after fertilization, the eggs were transferred to seawater at 4°C for 20 minutes of cold shock, so that the grouper gynogenetic haploid chromosome group doubled and became gynogenetic diploid. The treated grouper gynogenetic eggs are transferred into norma...

Embodiment 2

[0022] Similar to Example 1, the difference is that the grouper semen is diluted 10 times with the diluent, placed in a petri dish with a diameter of 9-15 cm, and irradiated with ultraviolet light at a dose of 500×100 μW / cm 2 ; Genetic inactivation of spermatozoa. Mix processed sperm with eggs to a final sperm concentration of at least 10 5 per ml, add seawater at a temperature of 25°C to start the gynogenesis of grouper eggs. Three minutes after fertilization, the eggs were transferred to seawater at 6°C for cold shock for 25 minutes, so that the grouper gynogenetic haploid chromosome group doubled and became gynogenetic diploid. The treated grouper gynogenetic eggs are transferred into normal water temperature seawater and managed as ordinary grouper fry to produce grouper gynogenetic diploid fry.

Embodiment 3

[0024] Similar to Example 1, the difference is that the grouper semen is diluted 8 times with the diluent, placed in a petri dish with a diameter of 9-15 cm, and irradiated with ultraviolet light at a dose of 700×100 μW / cm 2 ; Genetic inactivation of spermatozoa. Mix processed sperm with eggs to a final sperm concentration of at least 10 5 per ml, add seawater at a temperature of 22°C to start the gynogenesis of grouper eggs. Three minutes after fertilization, the eggs were transferred to seawater at 3°C ​​for cold shock for 15 minutes to double the grouper gynogenetic haploid chromosome set and become gynogenetic diploid. The treated grouper gynogenetic eggs are transferred into normal water temperature seawater and managed as ordinary grouper fry to produce grouper gynogenetic diploid fry.

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Abstract

The invention discloses a method for inducing gynogenetic diploid of grouper, which comprises the following steps of: diluting semen of the grouper, and radiating the semen by using ultraviolet rays to make the heritability of the semen deactivated; mixing with mature eggs of the grouper; after the eggs are fertilized, transferring to seawater for cold shock treatment to double genomes and generate the gynogenetic diploid; and transferring to seawater, and managing according to common grouper fry to obtain the gynogenetic diploid of the grouper. The offspring generated by induction only contains the gene of the female parent, the homozygosis of good genes can be quickened, and good hereditary features are quickly fixed; and the method can be applied to genetic analysis of the grouper, and has wide application prospect in the aspects of culture and breeding of the grouper.

Description

technical field [0001] The invention relates to the field of fish genetics and breeding, in particular to a method for inducing grouper gynogenetic diploids. Background technique [0002] Gynogenesis is a type of parthenogenesis, which refers to the reproductive behavior in which an egg develops into an individual only by its own genetic material. The same or heterogeneous sperm entering the egg only stimulates the development of the egg and does not participate in the development. The genetic material of the offspring is completely derived from the gynonucleus, and the embryonic development is completely under the control of the gynogenetic nucleus. The offspring only has the female parent. traits. [0003] There are 4-5 species of natural gynogenesis in fishes, such as Heilongjiang gibel crucian carp, Dianchi high-backed crucian carp, Taiwan Mori's genus, and Asian hook-toothed butterfly. Artificially inducing gynogenesis is one of the most effective methods to accelerat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张勇张海发黄文周翰林卢丹琪李水生蒙子宁刘晓春林浩然
Owner SUN YAT SEN UNIV
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