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Method for inducing gynogenesis of cyprinus carpio L. by using megalobrama amblycephala sperms

A technology for gynogenesis and gynogenesis of typhoid bream is applied in the field of gynogenesis of koi carp induced by bream bream sperm, which can solve the problems of promiscuity, unstable genetic traits, unclear genetic mechanism of koi pigments, etc., and achieve accelerated gene homozygosity. the effect of

Inactive Publication Date: 2013-02-13
BEIJING FISHERIES RES INST
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Problems solved by technology

This fish is bred by artificial hybridization of mutant carp strains. The genes are highly mixed and the genetic traits are extremely unstable. During the breeding process, the phenotypic characteristics of color spots are mainly used for selection, while the market is mainly based on its flower color, Stripes are the main ornamental value, but the genetic mechanism of koi pigment is still unclear

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Embodiment Construction

[0012] Below in conjunction with specific embodiment the present invention is described in further detail:

[0013] 2 to 3 months before the breeding season, select female koi with neat color spots, strong breed characteristics, excellent color quality, good body shape, health and disease-free, and obvious sexual maturity characteristics, and male groups with obvious sexual maturity characteristics and healthy physique. As a broodstock, the bream was intensively raised in a special pond, and the broodstock compound feed added with Ve was selected for reasonable feeding to promote the good development of gonads.

[0014] When the water temperature is stable at 20-22°C, select the above-mentioned parent fish with good gonad development and strong physique, and select male koi with 2 to 3 times the number of female koi (for stimulating female koi to estrus), and artificially induce labor. Mixed injection LRH-A 2 20μg / Kg, HCG1000IU / Kg, the dosage of male fish is halved, and the m...

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Abstract

The invention discloses a method for inducing gynogenesis of cyprinus carpio L. by using megalobrama amblycephala sperms. The method comprises the following steps of: firstly, selecting a female gynogenesis of cyprinus carpio L. and a male megalobrama amblycephala as parent fishes, secondly, performing artificial induced spawning to obtain megalobrama amblycephala sperms and cyprinus carpio L. spawns; and finally, uniformly mixing ultraviolet-inactivated megalobrama amblycephala seminal sperms and the cyprinus carpio L. spawns, scattering the mixture on a mesh which is flatly paved in water, after 5 minutes, placing activated spawns in cold water of 5 DEG C to perform cold shock treatment for 18 to 25 minutes, and incubating embryos after the cold shock treatment at 21 to 23 DEG C in water to obtain diploid gynogenesis cyprinus carpio L. According to the method, the measures of activating the gynogenesis of the cyprinus carpio L. spawns by using the inactivated megalobrama amblycephala seminal sperms and doubling genomes are used, so that the induced descendants only contain genes of female parents, the homozygosis of genes can be accelerated, and the method has important biological significance in the aspects of color inheritance law study and genetic breeding of the cyprinus carpio L.

Description

technical field [0001] The invention belongs to the technical field of fish cell engineering breeding, and in particular relates to a method for inducing koi gynogenesis by bream sperm. Background technique [0002] Gynogenesis is a type of parthenogenesis, which refers to the reproductive behavior in which an egg develops into an individual only by relying on its own genetic material. In fish purification and breeding, it is generally believed that the homozygous effect of one generation of gynogenetic fish is equivalent to the self-purification effect of multiple generations of fish, so high genetic purity, strong stress resistance, and growth can be obtained in the offspring of gynogenetic fish. Advantageous individual such as speed. As a cell engineering technology, artificial gynogenesis technology has important theoretical significance and broad application prospects in fish purification and breeding, exploration of fish sex determination mechanism, rapid establishmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 梁拥军张升利杨璞孙向军史东杰张欣苏建通孙砚胜
Owner BEIJING FISHERIES RES INST
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