Method for breeding colored rice two-line hybrid rice
A technology of hybrid rice and colored rice, which is applied in the field of breeding of two-line hybrid rice of colored rice, can solve the problems of no advantage in yield and quality, restriction of production and consumer application, and decline of functional nutrition, so as to shorten the breeding time and reduce the The effect of production promotion risk and reduction of transfer time
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[0042] A method for breeding colored rice two-line hybrid rice, the specific steps are as follows:
[0043] (1) Population construction: Indica black rice line Zhongze Heizhen (ie: colored rice +C ) as the male parent, Guangzhan 63-4S (i.e. two-line male sterile line S) as the female parent, and the offspring obtained were backcrossed with Guangzhan 63-4S for 4 times to obtain BC 4 f 1 2 generation plants, BC 4 f 1 Generation selfing 1 time, harvest BC 4 f 2 substitute seeds;
[0044] (2) Select test cross: observe BC 4 f 2 A total of 1202 generation seeds were selected, and homozygous seeds with colored seed coats were selected and planted as a single plant, and a total of 322 plants were planted; when planting to flowering, 53 male sterile plants among the 322 plants were selected to obtain the male sterile plant S ' 1 ; The male sterile plant S' 1 Paired test crossing with Yangdao 6 (i.e. restorer line R) to obtain hybrid F 1 generation;
[0045] Then 53 male st...
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