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Self-compatibility method of potatoes

A potato and compatibility technology, applied in the field of genetic breeding, can solve problems such as high content of toxic substance solanine, diploid potato self-incompatibility, and excessively long stolons

Active Publication Date: 2019-04-02
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Sli gene comes from wild potatoes, and its introduction into cultivated species often leads to many undesirable traits, such as excessively long stolons (greater than 1 meter), high levels of toxic substance solanine, etc.
Moreover, these undesirable traits are controlled by polygenes, and it is difficult to eliminate them in actual breeding work
Therefore, the problem to be solved in this area is how to overcome the difficult problem of diploid potato self-incompatibility

Method used

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Embodiment 1

[0040] Embodiment one: a kind of method that the present invention discloses makes potato self-compatibility, comprises the steps:

[0041] (1) Through artificial self-pollination at the flowering stage of more than 200 diploid potatoes, one self-compatibility material was screened out, which was recorded as PG6359, and the S-RNase gene in PG6359 was cloned by transcriptome sequencing;

[0042] (2) The S-RNase gene cloned in the step (1) obtains two full-length sequences of the S-RNase gene, which are respectively denoted as S s11 , S s12 , S s11 The gene sequence is shown in SEQ ID No.1, S s12 The gene sequence of the gene sequence is shown in SEQ ID NO.2. After artificial self-pollination of the material PG6359, the genotype in the selected progeny is S s11 S s11 as the female parent, the female parent is recorded as material A, a self-incompatible material is used as the male parent, and the male parent material is recorded as material B, and F is obtained by hybridizat...

Embodiment 2

[0045] Embodiment two: a kind of method that the present invention discloses makes potato self-compatibility, comprises the steps:

[0046] (1) Screen out the potato self-compatibility material, which is denoted as PG6359, and the S-RNase gene in PG6359 is cloned by the transcriptome sequencing method;

[0047] (2) The S-RNase gene cloned in the step (1) obtains two full-length sequences of the S-RNase gene, which are respectively denoted as S s11 , S s12 , S s11 The gene sequence is shown in SEQ ID No.1, S s12 The gene sequence of the gene sequence is shown in SEQ ID NO.2. After artificial self-pollination of the material PG6359, the genotype in the selected progeny is S s11 S s11 As the female parent, the female parent is recorded as material A, and a self-incompatible material is used as the male parent, and the male parent material is recorded as material B. The F1 generation is obtained by crossing, and the genotype of the F1 generation is determined. F1 generation i...

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Abstract

The invention discloses a self-compatibility method of potatoes. The self-compatibility method comprises the following steps: (1) selecting a potato self-compatibility material, wherein the material is recorded as PG6359, and cloning S-RNase genes in PG6359 by virtue of a transcriptome sequencing method; and (2) acquiring two S-RNase gene full-length sequences which are respectively recorded as Ss11 and Ss12 from the cloned S-RNase genes in step (1), performing manual selfing pollination for the material PG6359, selecting a material with a genotype of Ss11Ss11 in a filial generation as a female parent, wherein the female parent is recorded as a material A, adopting a selfing non-compatible material as a male parent, wherein the male parent is recorded as a material B, and hybridizing to obtain a self-compatibility F1 generation. The self-compatibility method of the potatoes has the advantages that the self-compatibility of the diploid potato can be realized, and no wild potato fragmentis introduced, so that the linkage drag can be avoided, and a foundation is set for rapidly creating the selfing system of the diploid potato.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for making potatoes self-compatible. Background technique [0002] Potato has comprehensive nutrition and is the most important tuber food crop in the world. It plays an important role in solving the global food crisis. However, two structural obstacles have been restricting the sustainable development of the potato industry: 1) cultivated potatoes are mainly autotetraploid, and genetic analysis is very complicated, resulting in long breeding cycles; Breeding, there are disadvantages such as low reproduction coefficient (1:10), high seed cost and easy to carry diseases and insect pests. In this context, scientists in different countries have called for the redomestication of potatoes at the diploid level, the domestication of potatoes as seed-propagated crops. Compared with tetraploid potatoes, diploid potatoes have relatively simple genetics, and the coeffici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/158A01H6/827C12N9/22A01H1/022Y02A40/146A01H1/045A01H1/02
Inventor 黄三文张春芝杨中敏唐蝶
Owner AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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