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Breeding method for introducing corn parthenogernesis by using amiprofos-methyl

A methamidophos, parthenogenesis technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of high cost, difficult operation, low doubling rate, etc., and achieves high repeatability and operation. Easy, procedurally simple effects

Inactive Publication Date: 2013-05-08
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The technical problem to be solved in the present invention is: using the inhibitory effect of amisophos-methyl on cell division, inducing maize to directly form seeds through parthenogenety without double fertilization; Carcinogenic, disease-causing, high cost, difficult to operate, and problems such as unstable grain color development, difficult seed identification, complicated haploid doubling and low doubling rate in the Stock6 genetic induction method

Method used

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  • Breeding method for introducing corn parthenogernesis by using amiprofos-methyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Inducer preparation and treatment material selection

[0026] The marketed amisophos-methyl was selected and diluted with water to a concentration of 40 μmol / L inducer.

[0027] The object of treatment is the maize hybrid "Liaodan 565".

[0028] (2) Induction treatment

[0029] Ears are strictly bagged before silking. After most of the filaments on the fruit ear are spit out, cut the filaments short, and keep the length of the filaments at about 2.5 cm, then spray the filaments with a small sprayer, and put them back into the paper bag. Repeat the spraying twice a day at 6:00 and 8:00, respectively, for 2 consecutive days. 30 spikes were processed.

[0030] (3) harvest

[0031] The ears are harvested at maturity, the bracts are carefully peeled off, and the seeds are removed.

[0032] (4) Identification and reproduction

[0033] In the next season, the seeds will be sown as a single seed, and the individual plants that do not meet the breeding goals will be e...

Embodiment 2

[0035] (1) Inducer preparation and treatment material selection

[0036] The marketed amisophos-methyl was selected and diluted with water to a concentration of 60 μmol / L inducer.

[0037] The object of treatment is the maize hybrid "Zhengdan 958".

[0038] (2) Induction treatment

[0039] Ears are strictly bagged before silking. After most of the filaments on the ear are spit out, cut the filaments short, and keep the length of the filaments at about 2.0 cm, then use a small sprayer to spray the filaments, and finally put them back into the paper bag. The spraying was repeated 3 times a day at 6:00, 8:00 and 17:00, respectively, for 2 consecutive days. 40 spikes were processed.

[0040] (3) harvest

[0041] Harvest fruit ears after maturity, carefully peel off the bracts, take off the seeds, and obtain 180 seeds.

[0042] (4) Identification and reproduction

[0043] In the next season, the seeds were sown as a single seed, and a single plant with an excellent phenotype...

Embodiment 3

[0046] (1) Inducer preparation and treatment material selection

[0047] Select the amisophos-methyl sold on the market, and dilute it with water to contain the inducer with a concentration of 80 μmol.

[0048] The processing object is "Jundan 20".

[0049] (2) Induction treatment

[0050] Planted under the condition of 1000 meters of space isolation, strictly emasculated before powder loosening. When most of the filaments on the ear are spit out and the length of the filaments is about 2.0 cm, use a sprayer to spray the filaments. Repeat the spraying 3 times at 6:00, 8:00 and 16:00 for 2 consecutive days. 50 spikes were processed.

[0051] (3) harvest

[0052] Harvest fruit ears after maturity, carefully peel off the bracts, remove the seeds, and harvest 200 seeds.

[0053] (4) Identification and reproduction

[0054] In the next season, the seeds will be sown as a single seed, and 150 excellent single plants will be selected according to the phenotype, and 100 ears wi...

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Abstract

The invention relates to a breeding method for introducing corn parthenogernesis by using amiprofos-methyl, and belongs to the field of planting breeding. The method comprises the following steps of: preparing an inductive agent of which the concentration is 5-200mumol / L from the amiprofos-methyl and water; performing introducing treatment: when filaments sprout to grow to about 2.5mm, spraying or daubing the inductive agent to the filaments, and harvesting to obtain homozygous genotype diploid seeds when seeds are mature; and during seeding in the next season, selecting and identifying by using the conventional method to select head progeny rows according with the breeding aim to obtain a homozygous corn selfing line. The breeding method for introducing the corn parthenogernesis by using the amiprofos-methyl has the positive effects of avoiding the artificial pollination, and being simple in method, easy to operate, low in cost, high in efficiency, and capable of being applied in large scale; in addition, the diploid seeds are directly formed and a chromosome doubling process is not required; and in the breeding method, more than 5 generations are shortened in comparison with a breeding method of the conventional corn selfing line.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to a breeding method for corn inbred lines. Background technique [0002] In the conventional method of breeding maize inbred lines, at least 7 selfing generations are required to complete the breeding process of an inbred line, and more generations are required for materials with complex genetic backgrounds. In order to shorten the breeding cycle, the existing method at home and abroad is to use the Stock6 induction line to carry out genetic induction, after inducing haploid seeds, and then use colchicine and other chromosome doubling agents to double them into diploids; Chemical reagents such as narcissine treat filaments to induce maize to directly produce homozygous diploid seeds through parthenogenesis. However, these methods have the following disadvantages: [0003] 1. After induction by the Stock6 genetic induction method, each seed needs to be identified to find ou...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 朱敏史振声李凤海王志斌王宏伟吕香玲葛云侠
Owner SHENYANG AGRI UNIV
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