New corn haploid inducing line and applications thereof

Abstract

The present invention belongs to the technical field of gene engineering, and specially relates to a new corn haploid inducing line and applications thereof. According to the present invention, transgenic corn expressing a fluorescent protein gene, an insect-resistant gene and anti-weeding-agent gene is adopted as the male parent (male), and is subjected to hybridization with a haploid inducing line female parent (female) to generate a hybridization generation, and multiple back cross, screening and selfing are performed to obtain the new corn haploid inducing line; the problems that the haploid is difficult to identify, the accuracy is not high, the speed is slow, the identifying is dependent on experienced researchers and the like in the previous system are solved with the new corn haploid inducing line of the present invention; the rapid identifying can be performed with the simple device without the experienced researchers, the accuracy is high, the time and the labor are saved, the large-scale haploid production can be achieved, and the new corn haploid inducing line is used for corn breeding; and the new corn haploid inducing line has characteristics of insect resistance and weeding agent resistance, is beneficial for field management and improvement of the quantity and the quality of haploid induction.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the generation and application of a new maize haploid induction line. Background technique [0002] It is well known that haploid plant genomes contain only one set of chromosomes and are therefore usually sterile. But through natural chromosome doubling or artificial doubling, double haploids can be formed. Since chromosome doubling is the doubling of the genome of a haploid plant, the two chromosome sets of the resulting diploid plant are identical (homogeneous). This is distinguished from heterozygous diploids (one chromosome set from the mother and one from the father) formed by crossing. Because the double haploid is completely homogeneous, it is a good inbred line material in plant breeding and can be directly used to prepare hybrid combinations. [0003] In the past, plant breeders have primarily bred through selection and crossbreeding. In plan...

AI Technical Summary

Problems solved by technology

However, the application of this system is affected by the following factors: 1. The induced maternal material cannot have pigment expression, because this will mask the R1-nj marker; 2. The expression of the R1-nj gene is affected by environmental factors (Kebede et al .,2011; Prigge et al.,2011;Prigge et al.,2012; 2005); 3. The expression of R1-nj is affected by other anthocyanin synthesis and regulation genes, such as c1-I, c2-Idf and in-1D (Burr et al., 1996; DellaVedova et al., 2005; Paz -Ares et al.,1990); 4. The identification of haploid often requires experienced personnel to identify

To overcome the shortcomings of the R1-nj system, it was proposed to use seed oil content or B1 and PL1 markers as alternative screening markers, but these systems are not stable and accurate (Rotarenco et al., 2010; Rotarenco et al., 2007)

[0007] In addition, most of the current haploid induction lines are not resistant to insects and herbicides. Therefore, during the growth and hybridization of the induction lines, weed management is difficult, and insect damage is prone to occur, which affects the reproduction of the induction lines and the induction of haploids.

Images