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Molecule marking method of rice blast-resisting gene

A rice blast resistance gene and molecular marker technology, applied in the field of molecular genetics and breeding of crops, can solve the problems of difficult disease resistance gene aggregation, difficult phenotype identification, low breeding efficiency, etc., and achieves convenient identification, control of breeding population size and cost. high effect

Inactive Publication Date: 2011-08-24
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

Method used

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  • Molecule marking method of rice blast-resisting gene
  • Molecule marking method of rice blast-resisting gene
  • Molecule marking method of rice blast-resisting gene

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Embodiment 1

[0024] (1) Materials and methods:

[0025] 1. Li Peifu et al. used blast-resistant landrace rice variety Bodiao (♀) and susceptible variety Su Yunuo ( ) to get F 1 , selfing to obtain F 2 Isolate populations for genetic analysis. It is clear that the resistance of Oryza sativa to the "Bei 1" strain is controlled by a pair of major dominant genes, and further allelism is carried out by crossing Oryza sativa with 12 differentiating cultivars of rice blast in Japan The results showed that the resistance gene of thin rice to the North 1 strain was non-allelic to the known disease resistance genes carried by the 12 differentiated varieties, and the gene was named Pi-bd1(t) (Heritage, 2007 , 29(10): 1249-1255). On this basis, the present invention adopts single-grain transmission method to construct F 2∶6 Recombinant inbred line population, that is, two parents are crossed to obtain F 1 , selfing to obtain F 2 , F 2 Randomly select 166 individual plants, self-fertilize each ...

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Abstract

The invention discloses a molecule marking method of a rice blast-resisting gene, belonging to the field of crop molecule heredity and breeding. The method comprises the following steps: (1) taking a rice sample and extracting a genome DNA (Deoxyribose Nucleic Acid) of the rice sample; and (2) carrying out PCR (Polymerase Chain Reaction) amplification on the genome DNA of the rice sample by utilizing any one pair of molecule-marked primers in RM6091 and RM26632, carrying out electrophoresis detection on a PCR amplification product, and if a molecule-marked DNA segment with corresponding size is amplified, showing that a Pi-bdl(t) gene exists. Through rice blast-resisting gene Pi-bdl(t) molecule marking in the invention, whether thin rice as well as crossbred descendants, backcross descendants and multiple cross descendants thereof contain the gene can be detected, the resistance level of the gene on rice blast can be forecasted, the selecting efficiency of rice blast resistant materials can be greatly increased, and the breeding process for disease resistance can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a molecular marker method for a rice blast resistance gene. Background technique [0002] Rice is one of the most important food crops in our country, which is of great significance to ensure our country's food security and national economic growth. Rice blast is the most serious disease in my country's rice production, and has the characteristics of fast transmission, wide occurrence, and heavy damage (Ling Zhongzhuan, etc., 1989, Research on Physiological Races of Magnaporthe oryzae in Northern Rice Regions of my country, Chongguo Agricultural Sciences, 22(3 ):7-13). It is the most economical and effective way to control the occurrence of rice blast and reduce the loss of rice yield by further exploring and utilizing the genetic resources of rice blast resistance in my country and cultivating and planting disease-resistant varieties. [0003] So far, scientist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张红生王建飞鲍永美环晶黄骥王州飞
Owner NANJING AGRICULTURAL UNIVERSITY
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