Molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy

Abstract

The invention belongs to the field of molecular genetics, and discloses a molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy. The method comprises the following steps of: (1) selecting a paddy sample, and extracting genome deoxyribonucleic acid (DNA) of the paddy sample; and (2) performing polymerase chain reaction (PCR) amplification on the genome DNA of the paddy sample by utilizing any one, two or three pairs of molecular marker primers in s01, in5 and in3, performing electrophoretic detection on a PCR amplification product, and if corresponding DNA fragments of molecularmarkers are amplified, indicating that the Pi-hk1 (t) gene exists. In the method, the selection efficiency of a single marker is over 99.92 percent, and the selection efficiency of any double markersis 100 percent. The resistance level of the gene on rice blast can be forecasted by detecting whether the paddy contains the gene or not by the molecular marker primers provided by the invention, so the selection efficiency of the rice blast resistance of the paddy is improved greatly, and the process for breeding for disease resistance is accelerated.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker method of the rice blast resistance gene Pi-hk1(t), in particular to a molecular marker method of the rice blast resistance gene Pi-hk1(t) of A. Molecular marker-assisted selective breeding of rice blast varieties and utilization of germplasm resources. Background technique [0002] Rice is an important food crop in my country. Rice blast caused by blast fungus is one of the most threatening diseases to rice production, and it occurs in rice production areas. It is estimated that the global annual damage caused by rice blast is about billions of dollars in losses (Jeon et al, 2003). my country loses hundreds of millions of kilograms of rice every year due to rice blast damage. In the popular years of rice blast, the yield loss caused is generally 10-20%, and can reach more than 50% in severe cases. Some fields even fail to harvest, and cause the quality of ri...

AI Technical Summary

Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

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