A molecular marker closely linked to rice race-specific blast resistance gene and its application

Abstract

The invention belongs to the field of crop molecular genetics and breeding, and discloses a molecular marker closely linked with a race-specific rice-blast-resistant gene of paddy rice and application of the molecular marker. A marking method comprises the following steps: (1) taking a paddy rice sample and extracting genomic DNA of the paddy rice sample; (2) performing PCR amplification on the genomic DNA of the paddy rice sample by utilizing any pair of primers of the molecular marker in RM24019 and RM24048, performing electrophoresis detection on a PCR amplification product, and if a DNA fragment of the molecular marker of the corresponding size is obtained by amplification, marking that the gene Pi-hk2(t) exists. Through the molecular marker closely linked with the race-specific rice-blast-resistant gene Pi-hk2 of the paddy rice, whether black-hull japonica rice as well as filial generation, backcross generation and multi-cross generation of the black-hull japonica rice contains the gene or not can be detected, the rice blast resistance level of the paddy rice can be predicted, the selection efficiency of a rice-blast-resistant material of the paddy rice is greatly improved, and the disease-resistant breeding process is accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a molecular marker closely linked with a rice race-specific blast resistance gene and an application thereof. Background technique [0002] Rice is one of the most important food crops in our country, which is of great significance to ensure our country's food security and national economic growth. Rice blast is the most serious disease in rice production in my country, and has the characteristics of rapid spread, wide occurrence, and severe damage (Ling Zhongzhuan et al., 1989, Research on the Physiological Race of Magnaporthe grisea in Northern my country. Agricultural Sciences of China, 22(3 ):7-13). It is the most economical and effective way to control the occurrence of rice blast and reduce the loss of rice yield by further exploring and utilizing the genetic resources of rice blast resistance in my country and cultivating and planting disease-resistant var...

AI Technical Summary

Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

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