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Molecule marking method for rice anti-rice blast gene Pigm

A rice blast resistance gene and molecular marker technology, applied in the field of molecular genetics and breeding of crops, can solve the problems of time-consuming and laborious, unreliable results, shortening the breeding cycle, etc., to improve the selection efficiency, facilitate identification, and control the size of the breeding population. Effect

Active Publication Date: 2014-11-05
YUAN LONGPING HIGH TECH AGRI CO LTD +2
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Conventional rice blast resistance breeding methods are time-consuming and labor-intensive, and the results are not very reliable. However, the use of molecular marker-assisted selection breeding is simple and effective, which can reduce breeding costs and shorten the breeding cycle. It can also carry out purposeful multi-gene aggregation and improve breeding efficiency. Bring huge social and economic benefits

Method used

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  • Molecule marking method for rice anti-rice blast gene Pigm
  • Molecule marking method for rice anti-rice blast gene Pigm
  • Molecule marking method for rice anti-rice blast gene Pigm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Specific molecular marker detection of broad-spectrum high blast resistance gene Pigm

[0037] 1 Experimental materials and sources

[0038]A total of 93 rice parent materials were selected for testing, among which 'Gumei No. 4' contained the Pigm gene, and the remaining 92 parents did not contain the Pigm gene. The specific materials and numbers are 1: Gumei No. 4; 2: 4517; 3: R998; 4: Minghui 86; 5: 9311; 6: Hua 4; 7: Huang Huazhan; 8: R207; 9: Zhongjian 15; 10 : YP16; 11: H819; 12: 3509; 13: Bolon; 14: 628S; 15: R792; 16: 4128S; 17: IR24; R2188; 23: 750S; 24: 3188; 25: 4127S; 26: 2537; 27: 9906; 28: 36S; 35: Minghui 63; 36: CBB23; 37: R08; 38: HY12; 39: R299; 40: Xiangwan No. 3; 41: Zaoyou 143; 42: 3509; 43: R624; 44: Ning 1013; Zhongzao 35;46:2855;47:2977;48:R402;49:ZR02;50:584;51:2729;52:R527;53:Zhongjian 17;54:H383;55:1517B;56:Zhongxiang Early 4; 57: Y-2; 58: R217; 59: 451; 60: IRRB23; 62: 2338; 63: H396; 64: YP13; 69: 3218; 70: 4217; 71: R608; 72: ...

Embodiment 2

[0049] Example 2 Correlation Analysis of Specific Molecular Marker Detection of Rice Blast Resistance Gene Pigm and Identification of Hybrid Combination Disease Nursery

[0050] 1 Experimental materials and sources

[0051] The BC of Gumei 4 / R527 / / R527 was obtained by crossing 'Gumei 4' and R527, and R527 was backcrossed once, and then self-crossed 1 f 2 Group, in the same way to obtain the BC of Gumei No. 4 / R228 / / R228, Gumei No. 4 / R624 / / R624 1 f 2 Groups, 46 individual plants were taken from different groups as research materials.

[0052] 2 Disease resistance identification

[0053] The population materials were planted in the disease nursery base of Dawei Mountain in Liuyang, Hunan Province, and the susceptibility and resistance of the materials were identified at the seedling stage by planting induced varieties. The results showed that the parent 'Gumei 4' was highly resistant, and R527, R228 and R624 were susceptible. Among the 46 hybrid plants of Gumei 4 / R527 / / R527 ...

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Abstract

The present invention provides a molecule marking method for a rice anti-rice blast gene Pigm, wherein four special molecule markers G1408, G2997, G2723 and G5301 are screened. According to the present invention, the four special molecule having co-segregation with the anti-rice blast gene are adopted to carry out PCR amplification and agarose gel electrophoresis detection on genome DNA of a rice parent strain or a hybridization generation, and the rice plant containing any one of the four special molecule markers after detection can be adopted as a breeding material containing high anti-rice blast Pigm gene. The method has characteristics of simple operation and accurate detection result, and can meet requirements of high-throughput molecule marker-assisted breeding.

Description

technical field [0001] The invention relates to the field of crop molecular genetics and breeding, in particular to a molecular marker method for rice blast resistance gene Pigm. Background technique [0002] Rice blast is a worldwide rice disease, and its damage area and damage degree are becoming more and more serious. It is called the cancer of rice. It is considered to be the most economical and effective method to control the damage of rice blast by using molecular breeding technology to select varieties containing rice blast resistance genes. Among the published rice blast resistance genes, the Pigm gene of the rice variety 'Gumei 4' is one of the genes with a broad spectrum of resistance and long-lasting resistance. All showed high resistance or immunity, and the effect was significantly better than that of the currently used broad-spectrum blast resistance genes Pi1, Pi2, Pi9, etc. After years of identification by the Hunan Rice Blast Disease Nursery, 'Gumei 4' has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/04
Inventor 戴小军杨远柱陈良碧胡小淳秦鹏符辰建
Owner YUAN LONGPING HIGH TECH AGRI CO LTD
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